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PMID: 2879225 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A novel RNA in which the 5' end is generated by cleavage at the poly(A) site of immunoglobulin heavy-chain secreted mRNA.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4749-52

Rogers J, Fasel N, Wall R

Abstract

We describe processed RNA species generated by cleavage at poly(A) sites in the immunoglobulin mu and gamma 2b heavy-chain transcription units. These "amputated transcripts" began at the first or "secreted" poly(A) site and ended at the second or "membrane" poly(A) site. Although they were polyadenylated and apparently spliced, they were largely restricted to the nucleus. Their existence confirms that the heavy-chain mRNAs are derived from RNA cleavage at alternative poly(A) sites.

MeSH Terms
B-Lymphocytes/immunology Base Sequence DNA Restriction Enzymes Genes Humans Immunoglobulin Heavy Chains/genetics Poly A/genetics RNA, Messenger/genetics Receptors, Antigen, B-Cell/genetics Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains RNA, Messenger Receptors, Antigen, B-Cell Poly A DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rogers J
Fasel N
Wall R
References (29)
29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4749-4752.1986
Published
1986-12-00
Pages
4749-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367262
Subset
IM
Grants
NCI NIH HHS · CA 12800 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4749-4752.1986 PMC Full Text View journal details
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