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PMID: 2884323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for the transit of aminopeptidase N through the basolateral membrane before it reaches the brush border of enterocytes.

The Journal of membrane biology ·Vol. 96 ·No. 1 ·1987-00-00 ·Pages 19-25

Massey D, Feracci H, Gorvel JP, Rigal A, Soulié JM, Maroux S

Abstract

In vivo pulse-chase labeling of rabbit jejunum loops was used in conjunction with subcellular fractionation and quantitative immunoprecipitation to determine whether or not the newly synthesized aminopeptidase N transits through the basolateral membrane before it reaches the apical brush border, its final localization. The kinetics of the arrival of the newly synthesized enzyme in the Golgi complex, basolateral and brush border membrane fractions strongly suggest that on leaving the Golgi aminopeptidase N is transiently integrated into the basolateral domain before reaching the brush border.

MeSH Terms
Aminopeptidases/metabolism Animals CD13 Antigens Cell Membrane/enzymology Intestinal Mucosa/enzymology Jejunum/enzymology Kinetics Microvilli/enzymology Rabbits Subcellular Fractions/enzymology
Chemicals
Aminopeptidases CD13 Antigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Massey D
Feracci H
Gorvel J P
Rigal A
Soulié J M
Maroux S
References (32)
32 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869331
Published
1987-00-00
Pages
19-25
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869331 View journal details
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