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PMID: 2898127 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human tyrosine hydroxylase and insulin genes are contiguous on chromosome 11.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4437-46

O'Malley KL, Rotwein P

Abstract

The gene for the catecholamine biosynthetic enzyme, tyrosine hydroxylase (TH), has been previously mapped to human chromosome 11 p15.5 in the vicinity of the loci for insulin (INS) and for the oncogene Harvey Ras 1 (HRAS). Here we show that gene probes derived from recombinant clones containing either human TH or INS cross-hybridize with each other. Direct DNA sequencing demonstrates that these genes are physically linked on chromosome 11. The TH gene is 5' to INS and is separated by only 2.7 kb of flanking DNA. Both genes have the same transcriptional polarity and form a head-to-tail linkage group with insulin-like growth factor 2 (IGF-2) in the order: 5' - TH - INS - IGF-2 - 3'. Because of the close physical proximity of these genes, previously described polymorphisms for INS are identical to those observed with TH. The localization of TH to the highly polymorphic INS locus provides four new restriction fragment length polymorphisms which should help determine rapidly whether defects in TH are responsible for bipolar affective disorder in the Old Order Amish and other populations.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Human, Pair 11 DNA Restriction Enzymes Genes Genetic Linkage Humans Insulin/genetics Molecular Sequence Data Nucleic Acid Hybridization Tyrosine 3-Monooxygenase/genetics
Chemicals
Insulin Tyrosine 3-Monooxygenase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Malley K L
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110.
Rotwein P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1988-05-25
Pages
4437-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336640
Subset
IM
Grants
NICHD NIH HHS · HD20805 · United States
NINDS NIH HHS · NS21318 · United States
PHS HHS · P437449 · United States
External Links
PubMed source PMC Full Text View journal details
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