Home LiteratureArticle Details
PMID: 2903501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8186-90

Sloan JH, Boss JM

Abstract

Class II major histocompatibility genes contain a conserved upstream sequence (CUS) that is important in the expression of these genes. This region has been divided into two major elements, the X box and the Y box. The ability of these elements to mediate transcription of a heterologous promoter was assayed upon transfection into a B-cell line (Raji), a class II-specific trans-acting factor-deficient B-cell line (RJ2.2.5 cells), and a T-cell line (Jurkat). The results showed that the X box element was responsible for directing tissue-specific expression when Raji cells were compared to Jurkat cells. The X box could not direct expression of the heterologous promoter in the trans-acting factor-deficient cell line, indicating that the X box is an ultimate target of the missing or defective factor in the RJ2.2.5 cell line. The Y box directed an equal but extremely low level of transcription in this system in both the mutant and wild-type B-cell lines, suggesting that this element is not involved in B-cell expression or as a target of the mutant factor.

MeSH Terms
B-Lymphocytes/metabolism Base Sequence Cell Line Gene Expression Regulation Genes, Homeobox Genes, MHC Class II Humans Mutation Transcription, Genetic Transfection
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sloan J H
Department of Microbiology and Immunology, Emory University, Atlanta, GA.
Boss J M
References (29)
29 references, click to expand
  1. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).
    J Natl Cancer Inst. 1966 Oct;37(4):547-59 PMID: 4288580
  2. Two distinct genetic loci regulating class II gene expression are defective in human mutant and patient cell lines.
    EMBO J. 1988 Jul;7(7):1965-72 PMID: 2458252
  3. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.
    J Exp Med. 1980 Dec 1;152(6):1709-19 PMID: 6778951
  4. Role of MHC gene products in immune regulation.
    Science. 1981 Jun 12;212(4500):1229-38 PMID: 6165083
  5. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
    Nature. 1981 Nov 19;294(5838):228-32 PMID: 6272123
  6. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  7. Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.
    J Exp Med. 1983 Mar 1;157(3):1053-8 PMID: 6403646
  8. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  9. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon.
    Nature. 1983 Oct 20-26;305(5936):726-9 PMID: 6415484
  10. The class II molecules of the human and murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):1-13 PMID: 6198089
  11. cis and trans activation of globin gene transcription in transient assays.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7428-32 PMID: 6200874
  12. Ia-negative B-cell variants reveal a coordinate regulation in the transcription of the HLA class II gene family.
    Immunogenetics. 1984;19(4):349-53 PMID: 6609124
  13. Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4917-21 PMID: 6431411
  14. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203 PMID: 6206493
  15. Gene organization of DC and DX subregions of the human major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3410-4 PMID: 3858830
  16. Reactivation by a trans-acting factor of human major histocompatibility complex Ia gene expression in interspecies hybrids between an Ia-negative human B-cell variant and an Ia-positive mouse B-cell lymphoma.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5145-9 PMID: 3875096
  17. Genetic complexity and expression of human class II histocompatibility antigens.
    Immunol Rev. 1985 Jul;85:45-86 PMID: 3899915
  18. Trans-acting element(s) operating across species barriers positively regulate expression of major histocompatibility complex class II genes.
    J Exp Med. 1985 Oct 1;162(4):1117-33 PMID: 3862745
  19. Evidence for two trans-acting genes regulating HLA class II antigen expression.
    J Immunol. 1985 Dec;135(6):4235-8 PMID: 3934280
  20. A trans-acting class II regulatory gene unlinked to the MHC controls expression of HLA class II genes.
    Nature. 1985 Nov 21-27;318(6043):291-3 PMID: 3934559
  21. trans activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment.
    J Virol. 1986 Mar;57(3):1016-22 PMID: 3005608
  22. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter.
    Nature. 1986 Feb 20-26;319(6055):640-6 PMID: 3005865
  23. Regulation of a transfected human class II major histocompatibility complex gene in human fibroblasts.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9139-43 PMID: 3097644
  24. Upstream DNA sequences required for tissue-specific expression of the HLA-DR alpha gene.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4254-8 PMID: 3495805
  25. A multiplicity of CCAAT box-binding proteins.
    Cell. 1987 Sep 11;50(6):863-72 PMID: 3476205
  26. Transcriptional enhancers in the HLA-DQ subregion.
    Mol Cell Biol. 1987 Sep;7(9):3315-9 PMID: 3118196
  27. Defective HLA class II expression in a regulatory mutant is partially complemented by activated ras oncogenes.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8603-7 PMID: 3317416
  28. In vitro RNA synthesis with SP6 RNA polymerase.
    Methods Enzymol. 1987;155:397-415 PMID: 2828872
  29. Stable variants affecting B cell alloantigens in human lymphoid cells.
    Nature. 1978 Feb 2;271(5644):459-61 PMID: 75508
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8186
Published
1988-11-00
Pages
8186-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282392
Subset
IM
Grants
NCRR NIH HHS · S07 RR05264 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.21.8186 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2