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PMID: 290997 Published · ppublish English Journal Article

Developmental and mutational changes of glycoproteins in the mouse neuronal retina: studies with bovine galactosyltransferase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3223-7

Wallenfels B

Abstract

Bovine galactosyltransferase (lactose synthase; EC 2.4.1.22) which catalyzes the transfer of galactose from UDPgalactose to glycoproteins with N-acetylglucosamine as the terminal residue of their oligosaccharide side chains was used to label glycoproteins of mouse retina with [14C]galactose. The glycoproteins were separated by isoelectric focusing in the first dimension and by sodium dodecyl sulfate gel electrophoresis in the second dimension. Their position on the gel was determined by autofluorography. With this method, quantitative as well as qualitative changes in the glycoprotein composition of the neuronal mouse retina during postnatal development were observed. Furthermore, it was found that the photoreceptor loss in mice with retinal degeneration was paralleled by the disappearance of certain glycoprotein bands.

MeSH Terms
Age Factors Animals Galactosyltransferases Glycoproteins/genetics,physiology Mice Mice, Inbred Strains Mutation Retina/growth & development,physiology Retinal Degeneration/genetics,physiopathology
Chemicals
Glycoproteins Galactosyltransferases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wallenfels B
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3223
Published
1979-07-00
Pages
3223-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383796
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3223 PMC Full Text View journal details
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