Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives.
Several synthetic 2'-O-methyl-RNA oligomers and their derivatives have been evaluated for inhibitory effect against HIV-induced cytopathic effect and expression of the virus specific antigen in cultured MT-4 cells. In this study, oligo(2'-O-methyl)ribonucleoside phosphorothioates showed a potent inhibitory activity with size dependency (25-mer showed it at 1 microM), but by contrast both 2'-O-methylribo- and deoxy-oligomers with normal phosphate linkages failed to inhibit. However, it should be noted that the patched oligo(2'-O-methyl)ribonucleotide (20-mer), in which five linkages at 5'- and three linkages at 3'-ends of normal phosphates were replaced with thiophosphates, has recovered the substantial inhibitory effect. These results show that the size of oligomer and phosphorothioate linkages, probably resistant to exolytic nucleases, are essential for exhibiting antiviral activity.
25 references, click to expand
-
Antisense oligodeoxynucleotides provide insight into mechanism of translation initiation of two Sendai virus mRNAs.J Biol Chem. 1987 Jun 5;262(16):7492-6 PMID: 2438279
-
Inhibition of rabbit globin mRNA translation by sequence-specific oligodeoxyribonucleotides.Biochemistry. 1985 Oct 22;24(22):6132-8 PMID: 4084510
-
Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides.Nucleic Acids Res. 1987 Aug 11;15(15):6131-48 PMID: 3627981
-
Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.Proc Natl Acad Sci U S A. 1987 Nov;84(21):7706-10 PMID: 3499613
-
Selective inhibition of the cytopathic effect of type A influenza viruses by oligodeoxynucleotides covalently linked to an intercalating agent.Nucleic Acids Res. 1987 Dec 10;15(23):9909-19 PMID: 3697085
-
Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylphosphonates in a cell-free system.Nucleic Acids Res. 1988 Apr 25;16(8):3341-58 PMID: 2836793
-
Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length.Nucleic Acids Res. 1986 Feb 11;14(3):1427-48 PMID: 3633502
-
Establishment of a high production system for AIDS retroviruses with a human T-leukemic cell line Molt-4.Cancer Lett. 1986 Mar;30(3):299-310 PMID: 2421869
-
Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.Proc Natl Acad Sci U S A. 1986 May;83(9):2787-91 PMID: 3010316
-
Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.Proc Natl Acad Sci U S A. 1986 Jun;83(12):4143-6 PMID: 3012555
-
Expression of human immunodeficiency virus antigen (HIV-Ag) in serum and cerebrospinal fluid during acute and chronic infection.Lancet. 1986 Jul 26;2(8500):177-80 PMID: 2873436
-
Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.Nucleic Acids Res. 1986 Jul 11;14(13):5399-407 PMID: 3737406
-
Inhibition of replication and cytopathic effect of human T cell lymphotropic virus type III/lymphadenopathy-associated virus by 3'-azido-3'-deoxythymidine in vitro.Antimicrob Agents Chemother. 1986 Dec;30(6):933-7 PMID: 2434024
-
Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H.FEBS Lett. 1987 May 11;215(2):327-30 PMID: 2438160
-
Physicochemical properties of phosphorothioate oligodeoxynucleotides.Nucleic Acids Res. 1988 Apr 25;16(8):3209-21 PMID: 2836790
-
Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.Proc Natl Acad Sci U S A. 1988 Feb;85(4):1028-32 PMID: 3277186
-
Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.Proc Natl Acad Sci U S A. 1988 Aug;85(15):5507-11 PMID: 3041414
-
A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors.Nucleic Acids Symp Ser. 1988;(19):117-20 PMID: 3226906
-
2'-O-Methyl polynucleotides as templates for cell-free amino acid incorporation.Biochemistry. 1971 Jun 22;10(13):2581-7 PMID: 4934115
-
Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide.Proc Natl Acad Sci U S A. 1978 Jan;75(1):280-4 PMID: 75545
-
Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide.Proc Natl Acad Sci U S A. 1978 Jan;75(1):285-8 PMID: 75546
-
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.Nucleic Acids Res. 1980 Nov 25;8(22):5473-89 PMID: 7465418
-
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.Nucleic Acids Res. 1984 Jun 11;12(11):4539-57 PMID: 6547529
-
Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay.Science. 1985 Aug 9;229(4713):563-6 PMID: 2992081
-
Site-directed cleavage of RNA.Nucleic Acids Res. 1987 Jun 11;15(11):4403-15 PMID: 2438655
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