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PMID: 2920027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A single-parameter family of adjustments for fitting enzyme kinetic models to progress-curve data.

The Biochemical journal ·Vol. 257 ·No. 1 ·1989-01-01 ·Pages 57-64

Duggleby RG, Nash JC

Abstract

Current methods for fitting integrated rate equations to enzyme progress curves treat each observation as if it were an independent measurement. When the data are obtained by taking several successive readings from each of a series of progress curves, the data will not be truly independent and will exhibit autocorrelation. Here we propose a simple pragmatic extension of integrated rate equations which takes account of first-order autocorrelations. The value of the method is assessed when applied to five sets of experimental data.

MeSH Terms
Enzymes/metabolism Kinetics Mathematics Models, Chemical
Chemicals
Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Duggleby R G
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Nash J C
References (10)
10 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2570057
Published
1989-01-01
Pages
57-64
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135537
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2570057 PMC Full Text View journal details
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