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PMID: 2925239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Oral administration of a streptococcal antigen coupled to cholera toxin B subunit evokes strong antibody responses in salivary glands and extramucosal tissues.

Infection and immunity ·Vol. 57 ·No. 4 ·1989-04-00 ·Pages 1072-7

Czerkinsky C, Russell MW, Lycke N, Lindblad M, Holmgren J

Abstract

Generation of local and systemic immune responses by the oral administration of antigens is frequently inefficient, requiring large quantities of immunogens and yielding only modest antibody responses. In this study, we have demonstrated that oral administration of microgram amounts of Streptococcus mutans protein antigen I/II covalently coupled to the B subunit of cholera toxin elicits vigorous mucosal as well as extramucosal immunoglobulin A and G antistreptococcal antibody responses in mice. These responses were manifested by the presence of large numbers of antibody-secreting cells in salivary glands, mesenteric lymph nodes, and spleens and by the development of high levels of circulating antibodies. This novel immunization strategy may find broad application in the construction of oral vaccines for the control of infectious diseases caused by pathogens encountered at mucosal and extramucosal sites.

MeSH Terms
Administration, Oral Animals Antibodies, Bacterial/biosynthesis Antibody-Producing Cells/physiology Antigens, Bacterial/administration & dosage Cholera Toxin/administration & dosage Female Immunoglobulin A/biosynthesis Immunoglobulin G/biosynthesis Mice Mice, Inbred C57BL Mucous Membrane/immunology Salivary Glands/immunology Spleen/immunology Streptococcus mutans/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Immunoglobulin A Immunoglobulin G Cholera Toxin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Czerkinsky C
Department of Medical Microbiology, University of Göteborg, Sweden.
Russell M W
Lycke N
Lindblad M
Holmgren J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.4.1072-1077.1989
Published
1989-04-00
Pages
1072-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313231
Subset
IM
Grants
NIDCR NIH HHS · DE 06746 · United States
External Links
PubMed source DOI: 10.1128/iai.57.4.1072-1077.1989 PMC Full Text View journal details
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