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PMID: 2935544 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A unique heparan sulfate in the nuclei of hepatocytes: structural changes with the growth state of the cells.

The Journal of cell biology ·Vol. 102 ·No. 2 ·1986-02-00 ·Pages 587-99

Fedarko NS, Conrad HE

Abstract

Growing and confluent cultures of a rat hepatocyte cell line were labeled with 35SO4(2-) and the heparan sulfate in the culture medium, the pericellular matrix, the nucleus, the nuclear outer membrane, and the remaining cytoplasmic pool was purified by DEAE-cellulose chromatography. The heparan sulfate in all pools from the confluent cells was bound more strongly on the DEAE-cellulose column than the corresponding pools from the growing cells. Gel filtration of each pool before and after beta-elimination showed that the heparan sulfate from the nuclear and nuclear membrane pools was composed of primarily free chains, whereas the heparan sulfate in all of the other pools was a mixture of proteoglycans and free chains. The heparan sulfate in each pool was cleaved with nitrous acid to obtain mixtures of di- and tetrasaccharides. Analysis of these mixtures showed that the structural features of the heparan sulfates in each pool were different and were altered significantly when the growing cells became confluent. The nuclear-plus-nuclear membrane pools represented 6.5% and 5.4% of the total cell-associated heparan sulfate in the growing cells and the confluent cells, respectively. The structural features of the heparan sulfate in the two nuclear pools were very similar to each other, but were markedly different from those of the heparan sulfate from the other pools or from any previously described heparan sulfate or heparin. The most unusual aspect of these structures was the high content of beta-D-glucuronosyl(2-SO4)----D-glucosamine-N,O-(SO4)2 disaccharide units in these sequences. The mode of biosynthesis and delivery of these unusual sequences to the nucleus and the potential significance of these observations are discussed.

MeSH Terms
Animals Cartilage/metabolism Cell Cycle Cell Nucleus/metabolism Chick Embryo Chondroitin Sulfates/metabolism Chromatography, High Pressure Liquid Glycosaminoglycans/metabolism Heparitin Sulfate/metabolism Liver/ultrastructure Oligosaccharides/metabolism Rats
Chemicals
Glycosaminoglycans Oligosaccharides Chondroitin Sulfates Heparitin Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fedarko N S
Conrad H E
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68 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.102.2.587
Published
1986-02-00
Pages
587-99
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114098
Subset
IM
Grants
NHLBI NIH HHS · HL 21763 · United States
External Links
PubMed source DOI: 10.1083/jcb.102.2.587 PMC Full Text View journal details
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