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PMID: 2944113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 18 ·1986-09-00 ·Pages 6716-20

Lundwall A, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlbäck B, Stenflo J, Wydro R

Abstract

Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/analysis,isolation & purification Epidermal Growth Factor/analysis Glycoproteins/analysis,genetics Humans Protein S RNA, Messenger/analysis
Chemicals
Glycoproteins Protein S RNA, Messenger Epidermal Growth Factor DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lundwall A
Dackowski W
Cohen E
Shaffer M
Mahr A
Dahlbäck B
Stenflo J
Wydro R
References (33)
33 references, click to expand
  1. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  2. Regulation of bovine activated protein C by protein S: the role of the cofactor protein in species specificity.
    Thromb Res. 1981 May 1;22(3):321-7 PMID: 6458116
  3. Purification of human vitamin K-dependent protein S and its limited proteolysis by thrombin.
    Biochem J. 1983 Mar 1;209(3):837-46 PMID: 6223624
  4. Purification of human C4b-binding protein and formation of its complex with vitamin K-dependent protein S.
    Biochem J. 1983 Mar 1;209(3):847-56 PMID: 6223625
  5. Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid.
    Biochemistry. 1983 Jun 7;22(12):2875-84 PMID: 6871167
  6. The occurrence of beta-hydroxyaspartic acid in the vitamin K-dependent blood coagulation zymogens.
    Biochem Biophys Res Commun. 1983 Aug 30;115(1):8-14 PMID: 6688526
  7. Beta-hydroxyaspartic acid in vitamin K-dependent proteins.
    J Biol Chem. 1983 Oct 25;258(20):12509-12 PMID: 6630196
  8. Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V.
    Blood. 1984 Feb;63(2):486-9 PMID: 6419799
  9. erythro-beta-Hydroxyaspartic acid in bovine factor IX and factor X.
    FEBS Lett. 1984 Jan 2;165(1):102-6 PMID: 6692907
  10. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  11. Computer-based characterization of epidermal growth factor precursor.
    Nature. 1984 Feb 9-15;307(5951):558-60 PMID: 6607417
  12. Characterization of a cDNA coding for human factor X.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3699-702 PMID: 6587384
  13. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  14. The gene structure of human anti-haemophilic factor IX.
    EMBO J. 1984 May;3(5):1053-60 PMID: 6329734
  15. Regulation of vitamin K-dependent protein S. Inactivation by thrombin.
    J Biol Chem. 1984 Aug 25;259(16):10335-9 PMID: 6236214
  16. Plasma protein S deficiency in familial thrombotic disease.
    Blood. 1984 Dec;64(6):1297-300 PMID: 6238642
  17. Familial protein S deficiency is associated with recurrent thrombosis.
    J Clin Invest. 1984 Dec;74(6):2082-8 PMID: 6239877
  18. Amino acid sequence of bovine protein Z: a vitamin K-dependent serine protease homolog.
    FEBS Lett. 1985 May 20;184(2):333-8 PMID: 3888670
  19. Characterization of an almost full-length cDNA coding for human blood coagulation factor X.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3591-5 PMID: 2582420
  20. The nucleotide sequence of the gene for human protein C.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4673-7 PMID: 2991887
  21. A powerful method for the preparation of cDNA libraries: isolation of cDNA encoding a 100-kDal nucleolar protein.
    Gene. 1985;37(1-3):215-20 PMID: 2996985
  22. Localization of thrombin cleavage sites in the amino-terminal region of bovine protein S.
    J Biol Chem. 1986 Apr 15;261(11):5111-5 PMID: 2937785
  23. Calcium binding of bovine protein S. Effect of thrombin cleavage and removal of the gamma-carboxyglutamic acid-containing region.
    J Biol Chem. 1986 Apr 15;261(11):5116-20 PMID: 2937786
  24. Primary structure of bovine vitamin K-dependent protein S.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4199-203 PMID: 2940598
  25. A comparison of human prothrombin, factor IX (Christmas factor), factor X (Stuart factor), and protein S.
    Biochemistry. 1977 Feb 22;16(4):698-706 PMID: 836809
  26. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  27. Characterization of protein S, a gamma-carboxyglutamic acid containing protein from bovine and human plasma.
    Biochemistry. 1979 Mar 6;18(5):899-904 PMID: 420821
  28. Protein S, a new vitamin K-dependent protein from bovine plasma.
    FEBS Lett. 1979 May 15;101(2):377-81 PMID: 109318
  29. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  30. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  31. High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2512-6 PMID: 6454142
  32. Regulation of activated protein C by protein S. The role of phospholipid in factor Va inactivation.
    J Biol Chem. 1981 Nov 10;256(21):11128-31 PMID: 6457049
  33. beta-Hydroxyaspartic acid in vitamin K-dependent protein C.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1802-6 PMID: 6572939
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.18.6716
Published
1986-09-00
Pages
6716-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386580
Subset
IM
Databases
GENBANK
M14338
External Links
PubMed source DOI: 10.1073/pnas.83.18.6716 PMC Full Text View journal details
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