Home LiteratureArticle Details
PMID: 2955901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal and neonatal rat heart.

Cell and tissue research ·Vol. 248 ·No. 3 ·1987-06-00 ·Pages 627-33

Toshimori H, Toshimori K, Oura C, Matsuo H

Abstract

An immunohistochemical study of atrial natriuretic polypeptides was carried out on embryonic, fetal and neonatal rat hearts, using an antiserum raised against alpha-human atrial natriuretic polypeptide (alpha-hANP). Weakly immunoreactive cells were seen in both atrial and ventricular walls at 11 days post coitum (pc). After this stage, the immunoreactive cells became more intensely stained in both atrial and ventricular walls. The immunoreactivity during the prenatal period was stronger in the superficial cell layer beneath the endocardium, than in the deep cell layer of the atrial wall. The cells in the trabecular meshwork also had an apparent, but weak, immunoreactivity, which showed a greater intensity in the left ventricle than in the right one. It is suggested that these immunoreactive cells in the ventricle may differentiate, in situ, into the cells of the impulse-conducting system during the further development of the heart.

MeSH Terms
Animals Animals, Newborn Atrial Natriuretic Factor/analysis Embryo, Mammalian Fetus Heart/embryology Immune Sera Immunoenzyme Techniques Microscopy, Electron Myocardium/cytology,ultrastructure Rats Rats, Inbred Strains
Chemicals
Immune Sera Atrial Natriuretic Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Toshimori H
Toshimori K
Oura C
Matsuo H
References (32)
32 references, click to expand
  1. Atrial natriuretic factor of the rat heart. Studies on isolation and properties.
    Proc Soc Exp Biol Med. 1982 Jun;170(2):133-8 PMID: 7201139
  2. Specific granules in mammalian and non-mammalian vertebrate cardiocytes.
    Methods Achiev Exp Pathol. 1971;5:173-213 PMID: 4949540
  3. Atriopeptin-immunoreactive neurons in the brain: presence in cardiovascular regulatory areas.
    Science. 1985 Mar 1;227(4690):1047-9 PMID: 2858127
  4. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
    J Histochem Cytochem. 1981 Apr;29(4):577-80 PMID: 6166661
  5. Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules.
    Can J Physiol Pharmacol. 1982 Mar;60(3):324-30 PMID: 7200387
  6. The development of Purkinje fibres and ordinary myocytes in the bovine fetal heart. An ultrastructural study.
    Anat Embryol (Berl). 1981;162(2):127-36 PMID: 7283176
  7. Structural identification of beta- and gamma-human atrial natriuretic polypeptides.
    Nature. 1985 Jan 31-Feb 6;313(6001):397-400 PMID: 3838202
  8. Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands.
    Histochemistry. 1984;80(2):113-27 PMID: 6370928
  9. Morphometric assessment of granulation in rat atrial cardiocytes: effect of age.
    J Mol Cell Cardiol. 1978 Aug;10(8):717-24 PMID: 702534
  10. The development of the conducting system in the heart of sheep.
    Br Heart J. 1951 Apr;13(2):129-47 PMID: 14821195
  11. Molecular forms of atrial natriuretic polypeptides in mammalian tissues and plasma.
    Biochem Biophys Res Commun. 1985 May 31;129(1):248-55 PMID: 4039933
  12. Immuohistochemical demonstration of alpha-atrial natriretic plypeptide-containing neurons in the rat brain.
    Histochemistry. 1985;83(1):1-3 PMID: 2931408
  13. Further studies about the microbodies in the atria of the heart.
    Exp Med Surg. 1965;23(1):1-12 PMID: 5844803
  14. Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria.
    Anat Embryol (Berl). 1984;170(2):123-7 PMID: 6549112
  15. Purification and complete amino acid sequence of alpha-human atrial natriuretic polypeptide (alpha-hANP).
    Biochem Biophys Res Commun. 1984 Jan 13;118(1):131-9 PMID: 6230082
  16. Functional and fine structural heterogeneity of atrial cardiocytes.
    Methods Achiev Exp Pathol. 1971;5:540-90 PMID: 4949548
  17. The development of the early atrioventricular conduction system in the embryonic heart.
    Can J Physiol Pharmacol. 1983 Aug;61(8):775-92 PMID: 6627122
  18. Evidence for the existence of atrial natriuretic factor-containing neurons in the rat brain.
    Neuroendocrinology. 1985 Jan;40(1):92-4 PMID: 3881691
  19. The use of avidin-biotin interaction in immunoenzymatic techniques.
    J Histochem Cytochem. 1979 Aug;27(8):1131-9 PMID: 90074
  20. SPECIFIC GRANULES IN ATRIAL MUSCLE CELLS.
    J Cell Biol. 1964 Oct;23:151-72 PMID: 14228508
  21. Developmental landmarks in cardiac morphogenesis: comparative chronology.
    Am J Cardiol. 1970 Feb;25(2):141-8 PMID: 5413193
  22. On the impulse conducting system of the monkey heart (Macaca mulatta). II. The atrio-ventricular node and bundle.
    Z Zellforsch Mikrosk Anat. 1973 Dec 6;145(3):363-88 PMID: 4204012
  23. Differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in the bovine heart: an immuno- and enzyme histochemical study.
    Histochem J. 1982 Nov;14(6):929-42 PMID: 6816763
  24. Identification in rat atrial tissue of multiple forms of natriuretic polypeptides of about 3,000 daltons.
    Biochem Biophys Res Commun. 1984 Jun 15;121(2):585-91 PMID: 6539598
  25. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats.
    Life Sci. 1981 Jan 5;28(1):89-94 PMID: 7219045
  26. Heart atria granularity effects of changes in water-electrolyte balance.
    Proc Soc Exp Biol Med. 1979 Sep;161(4):508-11 PMID: 482282
  27. Ultrastructural identification of human fetal Purkinje fibres--a comparative immunocytochemical and electron microscopic study of composition and structure of myofibrillar M-regions.
    J Mol Cell Cardiol. 1982 Aug;14(8):437-49 PMID: 6757450
  28. The right auricle of the heart is an endocrine organ. Cardiodilatin as a peptide hormone candidate.
    Anat Embryol (Berl). 1983;168(3):307-13 PMID: 6689515
  29. Development of specific granules in embryonic cardiac myocytes of the human auricular wall.
    Anat Embryol (Berl). 1980;160(1):1-9 PMID: 7469031
  30. The conduction system in the human heart at midgestation--immunohistochemical demonstration of the intermediate filament protein skeletin.
    Histochemistry. 1982;75(1):43-52 PMID: 6889590
  31. [The heart conduction tissue in the rat. Study by electron microscope. I. The common trunk of the bundle of His and the clear cells of the right auricle].
    Arch Mal Coeur Vaiss. 1959 Nov;52:1257-74 PMID: 13802412
  32. The auricular myocardiocytes of the heart constitute an endocrine organ. Characterization of a porcine cardiac peptide hormone, cardiodilatin-126.
    Cell Tissue Res. 1984;238(3):425-30 PMID: 6549270
Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00216493
Published
1987-06-00
Pages
627-33
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00216493 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2