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PMID: 2958493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Helper activity by human large granular lymphocytes in in vitro immunoglobulin synthesis.

Journal of clinical immunology ·Vol. 7 ·No. 5 ·1987-09-00 ·Pages 356-64

Rodriguez MA, Blanca I, Baroja ML, Arama S, Leon-Ponte M, Abadi I, Bianco NE

Abstract

In the present study we have examined the effect of human large granular lymphocytes (LGL) from healthy donors on Ig synthesis by autologous B lymphocytes. The results showed that this cell population has a consistent helper activity in pokeweed mitogen-activated cultures even when added at very low numbers. LGL can mediate their effect by secreting soluble helper factors capable of modulating B-cell responses as evidenced by the enhancement of IgG and IgM production by supernatants obtained from LGL cultures. Preincubation with interferon gamma further potentiated the helper activity by LGL.

MeSH Terms
Antibodies, Monoclonal B-Lymphocytes/immunology Cells, Cultured Humans Immunoglobulins/biosynthesis Interferon-gamma/immunology Lymphocyte Activation Lymphocytes/immunology T-Lymphocytes, Helper-Inducer/immunology
Chemicals
Antibodies, Monoclonal Immunoglobulins Interferon-gamma
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rodriguez M A
Centro Nacional de Enfermedades Reumaticas, Ministerio de Sanidad y Asistencia Social, Caracas, Venezuela.
Blanca I
Baroja M L
Arama S
Leon-Ponte M
Abadi I
Bianco N E
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Article Info
Journal
Journal of clinical immunology
Abbr.
J Clin Immunol
ISSN
0271-9142
DOI
10.1007/BF00917013
Published
1987-09-00
Pages
356-64
Language
English
Region
Netherlands
NLM ID
8102137
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00917013 View journal details
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