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PMID: 2960772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The subdivision of the T4 (CD4) subset on the basis of the differential expression of L-C/T200 antigens.

The Journal of experimental medicine ·Vol. 166 ·No. 6 ·1987-12-01 ·Pages 1758-73

Rudd CE, Morimoto C, Wong LL, Schlossman SF

Abstract

The T4 (CD4) subset of T lymphocytes has been subdivided into two major subsets, a suppressor/inducer subset (T4+,2H4+) and a helper subset (T4+,2H4-) on the basis of the differential expression of the L-C/T200 (CD45) antigens. The 2H4 antigen itself comprises at least three distinct polypeptides at 125,200, and 220 X 10(3) Mr, of which the 200 and 220 X 10(3) Mr polypeptides constitute the highest Mr isoforms of a pool of five distinct L-C/T200 antigens. The T4+,2H4+ subset expresses at least four of these isoforms at 180, 190, 200, and 220 X 10(3) on the cell surface, while the T4+,2H4- subset expresses only the 180 and 190 X 10(3) Mr forms. Pulse-chase analysis and endoglycosidase treatment revealed that the 125 X 10(3) Mr chain of the 2H4 antigen is nonglycosylated, while the 200 and 220 X 10(3) polypeptides are structurally related and derived by N- and O-linked glycosylation from two nascent subunits at 150 and 160 X 10(3) Mr. The function of the T4+,2H4+ subset could be blocked only by an antibody reactive with the L-C/T200 isoforms enriched with O-linked oligosaccharides at 200 and 220 X 10(3) Mr.

MeSH Terms
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte/analysis Glycoproteins/immunology Glycoside Hydrolases Histocompatibility Antigens/immunology Humans Immune Tolerance Leukocyte Common Antigens Molecular Weight T-Lymphocytes/classification,immunology T-Lymphocytes, Helper-Inducer/classification T-Lymphocytes, Regulatory/classification
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Glycoproteins Histocompatibility Antigens Leukocyte Common Antigens Glycoside Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rudd C E
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Morimoto C
Wong L L
Schlossman S F
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.166.6.1758
Published
1987-12-01
Pages
1758-73
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188802
Subset
IM
Grants
NIAID NIH HHS · AI-12069 · United States
NIAID NIH HHS · AI-20729 · United States
NCI NIH HHS · CA-19589 · United States
External Links
PubMed source DOI: 10.1084/jem.166.6.1758 PMC Full Text View journal details
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