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PMID: 2964496 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CD16. Developmentally regulated IgG Fc receptors on cultured human monocytes.

The Journal of experimental medicine ·Vol. 167 ·No. 2 ·1988-02-01 ·Pages 408-20

Clarkson SB, Ory PA

Abstract

We have demonstrated that one Fc receptor for IgG (FcR) (CD16) on cultured human monocytes appears to be a developmentally regulated membrane protein. This receptor appears to contain less carbohydrate (if any) than does its counterpart on human neutrophils. Expression of CD16 on cultured monocytes increases with respect to both percentage of positive cells and numbers of sites per cell with length of time in culture. This was in contrast to expression of other types of FcRs that either decreased (CDw32) or did not change (FcRp72). Unlike an FcR that binds monomeric IgG (FcRp72), expression of CD16 on monocytes from most normal individuals was not influenced by IFN-gamma. After 14 d in culture, CD16 appeared to be the predominant FcR on cultured monocytes, and was capable of mediating both ligand attachment and phagocytosis. These findings support the hypothesis that CD16 plays an important role in mediating immunophagocytosis.

MeSH Terms
Antigens, Differentiation/analysis Antigens, Surface/analysis,immunology Cell Differentiation Cell Survival Cells, Cultured Humans Immunoglobulin G/metabolism Lymphocyte Function-Associated Antigen-1 Monocytes/classification,immunology,metabolism Neutrophils/immunology,metabolism Phagocytosis Phenotype Receptors, Fc/analysis,physiology Receptors, IgG
Chemicals
Antigens, Differentiation Antigens, Surface Immunoglobulin G Lymphocyte Function-Associated Antigen-1 Receptors, Fc Receptors, IgG
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clarkson S B
Rosalind Russell Arthritis Research Laboratory, University of California, San Francisco 94102.
Ory P A
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28 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.167.2.408
Published
1988-02-01
Pages
408-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188855
Subset
IM
Grants
NIAMS NIH HHS · AR-07348 · United States
External Links
PubMed source DOI: 10.1084/jem.167.2.408 PMC Full Text View journal details
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