Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 13 ·1988-07-00 ·Pages 4735-9

Silverman SJ, Sburlati A, Slater ML, Cabib E

Abstract

Previous work led to the puzzling conclusion that chitin synthase 1, the major chitin synthase activity in Saccharomyces cerevisiae, is not required for synthesis of the chitinous primary septum. The mechanism of in vivo synthesis of chitin has now been clarified by cloning the structural gene for the newly found chitin synthase 2, a relatively minor activity in yeast. Disruption of the chitin synthase 2 gene results in the loss of well-defined septa and in growth arrest, establishing that the gene product is essential for both septum formation and cell division.

MeSH Terms

Cell Division Cell Wall/metabolism Chitin Synthase/genetics,physiology Fungal Proteins/genetics,physiology Genes Genes, Fungal Glucosyltransferases/physiology Isoenzymes/genetics,physiology Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/enzymology,genetics,growth & development Schizosaccharomyces/metabolism Spores, Fungal

Chemicals

Fungal Proteins Isoenzymes Recombinant Proteins Glucosyltransferases Chitin Synthase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Silverman S J

Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.

Sburlati A

Slater M L

Cabib E

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.13.4735

Published

1988-07-00

Pages

4735-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC280510

Subset

External Links

PubMed source DOI: 10.1073/pnas.85.13.4735 PMC Full Text View journal details

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Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.

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