Home LiteratureArticle Details
PMID: 2971535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Thymocyte circular DNA excised from T cell receptor alpha-delta gene complex.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1669-74

Okazaki K, Sakano H

Abstract

We have characterized thymocyte circular DNA excised from the T cell receptor alpha-delta gene complex. Some delta gene clones contained unusual recombinant structures derived from V-(D)-J joining: (i) a reciprocal joint of direct V to J delta joining, skipping the D delta segment; (ii) a V-D delta coding joint lacking an adjacent D delta-J delta coding joint; (iii) a V- D structure containing two D delta segments. Many of the alpha gen clones contained both coding and reciprocal joints of V alpha-to-J alpha joining on the same structure. Most of these coding joints were out of phase; however, in one clone there was an in-phase V-J alpha structure. Interestingly, some alpha gene clones contained the same V gene sequence as rearranged in the delta gene clone, indicating that the same V gene family, at least in part, could be utilized for both the alpha and delta gene systems.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA, Circular/isolation & purification Genes Mice Mice, Inbred BALB C Molecular Sequence Data Receptors, Antigen, T-Cell/genetics Receptors, Antigen, T-Cell, alpha-beta Receptors, Antigen, T-Cell, gamma-delta Recombination, Genetic T-Lymphocytes/analysis Thymus Gland/analysis
Chemicals
DNA, Circular Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta Receptors, Antigen, T-Cell, gamma-delta
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Okazaki K
Department of Microbiology and Immunology, University of California, Berkeley 94720.
Sakano H
References (35)
35 references, click to expand
  1. Improved derivative of a phage lambda EK2 vector for cloning recombinant DNA.
    Nature. 1976 Oct 7;263(5577):526-7 PMID: 1067476
  2. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  3. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  4. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  5. In vitro packaging of lambda and cosmid DNA.
    Methods Enzymol. 1979;68:299-309 PMID: 161604
  6. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  7. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  8. Aberrant rearrangements contribute significantly to the allelic exclusion of immunoglobulin gene expression.
    Nature. 1981 Apr 2;290(5805):372-8 PMID: 6783959
  9. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.
    Nature. 1981 Apr 16;290(5807):562-5 PMID: 6783961
  10. Determination of nucleotide sequences in DNA.
    Science. 1981 Dec 11;214(4526):1205-10 PMID: 7302589
  11. Somatic recombination in a murine T-cell receptor gene.
    Nature. 1984 May 24-30;309(5966):322-6 PMID: 6328306
  12. Ordered rearrangement of immunoglobulin heavy chain variable region segments.
    EMBO J. 1984 Jun;3(6):1209-19 PMID: 6086308
  13. Developmentally controlled and tissue-specific expression of unrearranged VH gene segments.
    Cell. 1985 Feb;40(2):271-81 PMID: 2578321
  14. Chromosomal locations of the murine T-cell receptor alpha-chain gene and the T-cell gamma gene.
    Science. 1985 Feb 22;227(4689):941-5 PMID: 3918347
  15. The gene encoding the T-cell receptor alpha-chain maps close to the Np-2 locus on mouse chromosome 14.
    Nature. 1985 Mar 21-27;314(6008):271-3 PMID: 3920527
  16. Diversity and structure of genes of the alpha family of mouse T-cell antigen receptor.
    Nature. 1985 Aug 29-Sep 4;316(6031):783-7 PMID: 3839904
  17. Unusual organization and diversity of T-cell receptor alpha-chain genes.
    Nature. 1985 Aug 29-Sep 4;316(6031):828-32 PMID: 2993907
  18. Genomic organization of the genes encoding mouse T-cell receptor alpha-chain.
    Nature. 1985 Aug 29-Sep 4;316(6031):832-6 PMID: 2993908
  19. Molecular genetics of the T cell-receptor beta chain.
    Annu Rev Immunol. 1985;3:537-60 PMID: 3933533
  20. Two types of immunoglobulin-negative Abelson murine leukemia virus-transformed cells: implications for B-lymphocyte differentiation.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):145-9 PMID: 3001721
  21. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition.
    Annu Rev Immunol. 1986;4:529-91 PMID: 3518750
  22. A novel VH to VHDJH joining mechanism in heavy-chain-negative (null) pre-B cells results in heavy-chain production.
    Nature. 1986 Aug 28-Sep 3;322(6082):840-2 PMID: 3092105
  23. Recombination between an expressed immunoglobulin heavy-chain gene and a germline variable gene segment in a Ly 1+ B-cell lymphoma.
    Nature. 1986 Aug 28-Sep 3;322(6082):843-6 PMID: 3092106
  24. Differential expression of two distinct T-cell receptors during thymocyte development.
    Nature. 1987 Mar 5-11;326(6108):79-81 PMID: 3102971
  25. T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.
    Cell. 1987 May 22;49(4):477-85 PMID: 3502705
  26. Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.
    Nature. 1987 May 21-27;327(6119):242-3 PMID: 3033520
  27. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals.
    Cell. 1987 Jun 19;49(6):775-83 PMID: 3495343
  28. Structure, function, and serology of the T-cell antigen receptor complex.
    Annu Rev Immunol. 1987;5:503-40 PMID: 2439105
  29. A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation.
    Nature. 1987 Jun 25-Jul 1;327(6124):677-82 PMID: 2439914
  30. Identification and sequence of a fourth human T cell antigen receptor chain.
    Nature. 1987 Dec 10-16;330(6148):569-72 PMID: 2825032
  31. Peptide sequences of T-cell receptor delta and gamma chains are identical to predicted X and gamma proteins.
    Nature. 1987 Dec 10-16;330(6148):572-4 PMID: 3500416
  32. Delta is the Cx-gene product in the gamma/delta antigen receptor of dendritic epidermal cells.
    Nature. 1987 Dec 10-16;330(6148):574-6 PMID: 3500417
  33. Two pairs of recombination signals are sufficient to cause immunoglobulin V-(D)-J joining.
    Science. 1987 Nov 20;238(4830):1134-8 PMID: 3120312
  34. T-cell receptor delta gene rearrangements in early thymocytes.
    Nature. 1987 Dec 24-31;330(6150):722-7 PMID: 2961997
  35. The adult T-cell receptor delta-chain is diverse and distinct from that of fetal thymocytes.
    Nature. 1988 Feb 18;331(6157):627-31 PMID: 2963227
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1669-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457151
Subset
IM
Grants
NIAID NIH HHS · AI-18790 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2