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PMID: 2973057 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cleavage of the cII protein of phage lambda by purified HflA protease: control of the switch between lysis and lysogeny.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 7882-6

Cheng HH, Muhlrad PJ, Hoyt MA, Echols H

Abstract

The activity of the cII protein of phage lambda is probably the critical controlling factor in the choice of the lytic or lysogenic pathway by an infecting virus. Previous work has established that cII activity is regulated through the turnover of cII protein; the products of the hflA and hflB loci of Escherichia coli are needed for a degradative reaction, and lambda cIII functions in stabilizing cII. By using the cloned hflA locus, we have purified a cII-cleaving enzyme that we term HflA. Purified HflA contains three polypeptides; at least two of the subunits are products of the hflA region, and the third is probably a cleavage product of the larger of these two hflA-encoded polypeptides. The HflA protease activity cleaves cII to small fragments. We conclude that the switch between lambda developmental pathways involves regulated cleavage of cII by the specific protease HflA.

MeSH Terms
Bacteriophage lambda/analysis Cloning, Molecular Escherichia coli/enzymology,genetics Lysogeny Serine Endopeptidases/metabolism Transcription Factors/metabolism Viral Proteins
Chemicals
Transcription Factors Viral Proteins cII protein, bacteriophage lambda Hfla protease Serine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cheng H H
Department of Molecular Biology, University of California, Berkeley 94720.
Muhlrad P J
Hoyt M A
Echols H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.7882
Published
1988-11-00
Pages
7882-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282301
Subset
IM
Grants
NIGMS NIH HHS · GM 17078 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.21.7882 PMC Full Text View journal details
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