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PMID: 3279418 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 6 ·1988-03-00 ·Pages 1816-20

Nohmi T, Battista JR, Dodson LA, Walker GC

Abstract

The products of the SOS-regulated umuDC operon are required for most UV and chemical mutagenesis in Escherichia coli. It has been shown that the UmuD protein shares homology with LexA, the repressor of the SOS genes. In this paper we describe a series of genetic experiments that indicate that the purpose of RecA-mediated cleavage of UmuD at its bond between Cys-24 and Gly-25 is to activate UmuD for its role in mutagenesis and that the COOH-terminal fragment of UmuD is necessary and sufficient for the role of UmuD in UV mutagenesis. Other genetic experiments are presented that (i) support the hypothesis that the primary role of Ser-60 in UmuD function is to act as a nucleophile in the RecA-mediated cleavage reaction and (ii) raise the possibility that RecA has a third role in UV mutagenesis besides mediating the cleavage of LexA and UmuD.

MeSH Terms
Bacterial Proteins/genetics,metabolism DNA Damage DNA, Bacterial/analysis DNA-Directed DNA Polymerase Escherichia coli/drug effects,radiation effects Escherichia coli Proteins Mutation Peptide Fragments/analysis Plasmids Protein Processing, Post-Translational Rec A Recombinases/pharmacology Transcription, Genetic Ultraviolet Rays
Chemicals
Bacterial Proteins DNA, Bacterial Escherichia coli Proteins Peptide Fragments Rec A Recombinases DNA-Directed DNA Polymerase UmuD protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nohmi T
Biology Department, Massachusetts Institute of Technology, Cambridge 02139.
Battista J R
Dodson L A
Walker G C
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.6.1816
Published
1988-03-00
Pages
1816-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279870
Subset
IM
Grants
NCI NIH HHS · CA21615 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.6.1816 PMC Full Text View journal details
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