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PMID: 2981329 Published · ppublish English Journal Article

Middle tumor antigen of polyomavirus transformation-defective mutant NG59 is associated with pp60c-src.

Journal of virology ·Vol. 53 ·No. 1 ·1985-01-00 ·Pages 114-9

Bolen JB, Israel MA

Abstract

We have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant NG59 under gentle conditions that avoid ionic detergents results in detectable NG59-encoded middle tumor antigen (MTAg) associated with pp60c-src. This MTAg-pp60c-src complex could be immunoprecipitated from NG59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against MTAg. Immune complex kinase assays revealed that, whereas the pp60c-src associated with NG59 MTAg possessed tyrosyl kinase activity, the NG59 MTAg in this complex was not phosphorylated in these in vitro reactions. These results demonstrate that the point insertion mutation found in this transformation-deficient strain of polyomavirus encodes MTAg molecules capable of associating with pp60c-src and defines a limited region within MTAg which appears to be critical for stable MTAg-pp60c-src interactions.

MeSH Terms
Amino Acids/analysis Animals Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics,isolation & purification Cell Line Genes Genes, Viral Mice Mutation Oncogene Protein pp60(v-src) Peptide Fragments/analysis Phosphopeptides/analysis Polyomavirus/genetics Protein Kinases/genetics Viral Proteins/genetics,isolation & purification
Chemicals
Amino Acids Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Peptide Fragments Phosphopeptides Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bolen J B
Israel M A
References (35)
35 references, click to expand
  1. Heat-labile serum factor required for immunofluorescence of polyoma tumor antigens.
    Science. 1966 Sep 2;153(3740):1122-3 PMID: 4288238
  2. Polyoma virus transforming protein associates with the product of the c-src cellular gene.
    Nature. 1983 Jun 2-8;303(5916):435-9 PMID: 6304524
  3. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  4. Localization of gene functions in polyoma virus DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4169-73 PMID: 186787
  5. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  6. Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4666-70 PMID: 73183
  7. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):79-83 PMID: 203944
  8. Characterization of T antigens in polyoma-infected and transformed cells.
    Cell. 1978 Sep;15(1):65-77 PMID: 212199
  9. Identification of DNA sequence changes leading to loss of transforming ability in polyoma virus.
    J Biol Chem. 1980 Jan 10;255(1):230-5 PMID: 6243123
  10. Protein kinase activity associated with polyoma virus middle T antigen in vitro.
    Cell. 1979 Dec;18(4):915-24 PMID: 229972
  11. An activity phosphorylating tyrosine in polyoma T antigen immunoprecipitates.
    Cell. 1979 Dec;18(4):925-33 PMID: 229973
  12. Phosphorylation of polyoma T antigens.
    Cell. 1979 Dec;18(4):935-46 PMID: 229974
  13. Protein fingerprinting by SDS-gel electrophoresis after partial fragmentation with CNBr.
    Anal Biochem. 1979 Sep 1;97(2):382-6 PMID: 525799
  14. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  15. The hr-t gene of polyoma virus.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:263-70 PMID: 6253140
  16. Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein.
    Nature. 1981 Aug 13;292(5824):595-600 PMID: 6265803
  17. Characterization of polyoma mutants with altered middle and large T-antigens.
    J Virol. 1981 Sep;39(3):673-83 PMID: 6270343
  18. Comparison of phosphorylation of two polyoma virus middle T antigens in vivo and in vitro.
    J Virol. 1981 Oct;40(1):184-96 PMID: 6169848
  19. Purification of a tyrosine-specific protein kinase from Rous sarcoma virus-induced rat tumor.
    J Biol Chem. 1982 Jun 25;257(12):7135-42 PMID: 6282832
  20. Purification of polyoma virus medium-size tumor antigen by immunoaffinity chromatography.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4025-9 PMID: 6287461
  21. Peptide mapping of protein bands from polyacrylamide gel electrophoresis by chemical cleavage in gel pieces and re-electrophoresis.
    Anal Biochem. 1982 May 15;122(2):298-301 PMID: 7114449
  22. Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity.
    J Biol Chem. 1982 Nov 10;257(21):12467-70 PMID: 6290466
  23. Polyoma virus middle T antigen: relationship to cell membranes and apparent lack of ATP-binding activity.
    Mol Cell Biol. 1982 Oct;2(10):1187-98 PMID: 6184609
  24. Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.
    J Virol. 1983 Nov;48(2):352-60 PMID: 6312092
  25. Inhibition of polyoma virus middle T antigen-associated tyrosyl kinase activity by N-ethylmaleimide.
    J Biol Chem. 1983 Dec 25;258(24):15135-40 PMID: 6558075
  26. Construction and expression of a recombinant DNA gene encoding a polyomavirus middle-size tumor antigen with the carboxyl terminus of the vesicular stomatitis virus glycoprotein G.
    Mol Cell Biol. 1984 Feb;4(2):282-9 PMID: 6321957
  27. Host range mutants of polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):394-9 PMID: 4318787
  28. The complex of polyoma virus middle-T antigen and pp60c-src.
    EMBO J. 1984 Mar;3(3):585-91 PMID: 6325177
  29. Transmission of the polyoma virus middle T gene as the oncogene of a murine retrovirus.
    Nature. 1984 Apr 19-25;308(5961):748-50 PMID: 6325917
  30. In vitro association and phosphorylation of polyoma virus middle T antigen by cellular tyrosyl kinase activity.
    J Biol Chem. 1984 Oct 10;259(19):11686-94 PMID: 6434530
  31. Enhancement of cellular src gene product associated tyrosyl kinase activity following polyoma virus infection and transformation.
    Cell. 1984 Oct;38(3):767-77 PMID: 6091900
  32. The roles of individual polyoma virus early proteins in oncogenic transformation.
    Nature. 1982 Dec 23;300(5894):713-8 PMID: 6294529
  33. In vitro phosphorylation of angiotensin analogs by tyrosyl protein kinases.
    J Biol Chem. 1983 Jan 25;258(2):1022-5 PMID: 6822491
  34. Deletion mapping of a short polyoma virus middle T antigen segment important for transformation.
    J Virol. 1983 Apr;46(1):284-7 PMID: 6298462
  35. Polyoma middle-sized T antigen can be phosphorylated on tyrosine at multiple sites in vitro.
    EMBO J. 1984 Jan;3(1):73-9 PMID: 6200324
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.53.1.114-119.1985
Published
1985-01-00
Pages
114-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254986
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.53.1.114-119.1985 PMC Full Text View journal details
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