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PMID: 6325177 Published · ppublish English Journal Article

The complex of polyoma virus middle-T antigen and pp60c-src.

The EMBO journal ·Vol. 3 ·No. 3 ·1984-03-00 ·Pages 585-91

Courtneidge SA, Smith AE

Abstract

We have recently proposed that the transforming protein of polyoma virus, middle-T antigen, forms a complex with pp60c-src. Here we provide additional evidence for the existence of the complex using both monoclonal antibodies specific for middle-T and antibodies raised against synthetic peptides corresponding to sequences from both middle-T and pp60c-src. The complex was retained during partial purification of middle-T and was stable to incubation under various conditions. A survey of a number of mutants of middle-T antigen showed that there was a complete correlation between the ability of middle-T to accept phosphate in the in vitro kinase reaction and the presence of a middle-T: pp60c-src complex. This result is in accord with our hypothesis that middle-T itself is not a protein kinase but rather that pp60c-src phosphorylates middle-T. All mutant forms of middle-T antigen capable of transformation had associated pp60c-src. The middle-T of two non-transforming mutants (hr-t mutants) did not have associated pp60c-src, whereas other non-transforming middle-T species did associate with pp60c-src. We propose that the complex plays an essential role in transformation by polyoma virus, but that the existence of the complex per se may not be sufficient.

MeSH Terms
Antigens, Viral, Tumor/immunology,isolation & purification,metabolism Cell Transformation, Viral Cells, Cultured Macromolecular Substances Oncogene Protein pp60(v-src) Polyomavirus/physiology Protein Kinases/metabolism Viral Proteins/metabolism
Chemicals
Antigens, Viral, Tumor Macromolecular Substances Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Courtneidge S A
Smith A E
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-03-00
Pages
585-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557392
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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