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PMID: 6801651 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 12 ·1981-12-00 ·Pages 7412-6

Wong TW, Goldberg AR

Abstract

All the known avian sarcoma viruses have associated protein kinase activities that phosphorylate tyrosine residues of their target proteins. A decapeptide fragment of pp60src of Rous sarcoma virus (RSV), residues 415-424, and an analog of that sequence have been chemically synthesized by solid-phase methods. The two decapeptides were not phosphorylated by pp60src of RSV, P90 of Y73 avian sarcoma virus, or P140 of Fujinami sarcoma virus. However, both peptides were able to inhibit competitively the kinase activities associated with the transforming proteins. Antiserum was raised against one of the peptides and IgG was purified from the serum by affinity chromatography. The antibody was able to precipitate pp60src of RSV as well as P90 of Y73 virus from cells infected with these viruses. The antibody also precipitated a number of high molecular weight phosphoproteins from normal chicken and rat fibroblasts and from several lines of virus-transformed cells.

MeSH Terms
Animals Cells, Cultured Chick Embryo Cross Reactions Oncogene Protein pp60(v-src) Peptide Fragments/chemical synthesis,immunology,pharmacology Phosphoproteins/immunology Protein Kinase Inhibitors Protein Kinases/immunology Rats Viral Proteins/immunology
Chemicals
Peptide Fragments Phosphoproteins Protein Kinase Inhibitors Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wong T W
Goldberg A R
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.12.7412
Published
1981-12-00
Pages
7412-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349277
Subset
IM
Grants
NCI NIH HHS · CA13362 · United States
NCI NIH HHS · CA18213 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.12.7412 PMC Full Text View journal details
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