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PMID: 2981772 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A conjugative transposon (Tn919) in Streptococcus sanguis.

Infection and immunity ·Vol. 47 ·No. 2 ·1985-02-00 ·Pages 415-20

Fitzgerald GF, Clewell DB

Abstract

Streptococcus sanguis FC1, originally isolated from dental plaque, was found to be naturally resistant to tetracycline. Although no plasmid DNA could be detected, tetracycline resistance was transferable in filter matings to Streptococcus faecalis FA2-2. Again, no plasmid DNA was detectable in transconjugants, and the latter could donate tetracycline resistance to S. faecalis, S. sanguis, and Streptococcus lactis. The tetracycline resistance element was able to transpose to several sites on the S. faecalis hemolysin plasmid pAD1 and in each case resulted in a 15-kilobase insert. DNA filter blot hybridization studies showed that the element bears significant homology with the conjugative transposon Tn916. Designated Tn919, it was cloned into an Escherichia coli plasmid vector (pGL101) and, as has been shown for Tn916, excised readily in the absence of selective pressure.

MeSH Terms
Cloning, Molecular Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Streptococcus sanguis/analysis,drug effects,genetics Tetracycline/pharmacology
Chemicals
DNA Transposable Elements DNA, Bacterial Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fitzgerald G F
Clewell D B
References (33)
33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.47.2.415-420.1985
Published
1985-02-00
Pages
415-20
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263185
Subset
IM
Grants
NIAID NIH HHS · AI10318 · United States
NIDCR NIH HHS · DE02731 · United States
External Links
PubMed source DOI: 10.1128/iai.47.2.415-420.1985 PMC Full Text View journal details
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