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PMID: 2981802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene.

Journal of bacteriology ·Vol. 161 ·No. 1 ·1985-01-00 ·Pages 219-22

Thorbjarnardóttir S, Dingermann T, Rafnar T, Andrésson OS, Söll D, Eggertsson G

Abstract

We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene. The suppressor allele codes for a tRNA with anticodon CUA, presumably derived by a single base change from a CAA anticodon. The mature coding sequence of the leuX gene is preceded by a putative Pribnow box sequence (TATAAT) and followed by a termination signal. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5. The conserved nucleotides in these seven tRNAs recognized by E. coli leucyl-tRNA synthetase are located mainly in the aminoacyl stem and in the D-stem/loop region.

MeSH Terms
Anticodon Bacteriophage lambda/genetics Bacteriophages/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease HindIII Escherichia coli/genetics Genes, Bacterial Plasmids RNA, Transfer, Amino Acyl/genetics Suppression, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl DNA Restriction Enzymes Deoxyribonuclease HindIII
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Thorbjarnardóttir S
Dingermann T
Rafnar T
Andrésson O S
Söll D
Eggertsson G
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.1.219-222.1985
Published
1985-01-00
Pages
219-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214859
Subset
IM
Databases
GENBANK
M10747
External Links
PubMed source DOI: 10.1128/jb.161.1.219-222.1985 PMC Full Text View journal details
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