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PMID: 2981819 Published · ppublish English Journal Article

Regulation of a cya-lac fusion by cyclic AMP in Salmonella typhimurium.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 641-9

Jovanovich SB

Abstract

cya-lac and crp-lac operon fusions were isolated in Salmonella typhimurium by using the phage Mu d1(lac cts Apr). Both transduction and reversion analyses have indicated that lac expression is controlled by the appropriate promoter, e.g., either crpp or cyap. By using chromosomal mobilization techniques, we found that cya had a clockwise direction of transcription on the standard S. typhimurium map. The cya-lac fusions could be complemented by Escherichia coli F'133, which covers cya, with a resultant 17 to 38% decrease in cya expression. Cyclic AMP was found to be able to repress the expression of the cya-lac fusion ninefold when present at 25 mM. This repression was not seen in crp backgrounds, and hence is mediated by the cAMP receptor protein. Repression of cya was also found upon growth on carbon sources known to elicit high cyclic AMP levels.

MeSH Terms
Adenylyl Cyclases/genetics Bacterial Proteins/genetics Carbon/metabolism Culture Media Cyclic AMP/pharmacology Enzyme Activation Gene Expression Regulation Hemolysin Proteins Lac Operon Plasmids Salmonella typhimurium/genetics Transcription, Genetic
Chemicals
Bacterial Proteins CAMP protein, Streptococcus Culture Media Hemolysin Proteins Carbon Cyclic AMP Adenylyl Cyclases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jovanovich S B
References (42)
42 references, click to expand
  1. Structure of catabolite gene activator protein at 2.9-A resolution. Incorporation of amino acid sequence and interactions with cyclic AMP.
    J Biol Chem. 1982 Aug 25;257(16):9518-24 PMID: 6286624
  2. [The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].
    Biochim Biophys Acta. 1951 Nov;7(4):585-99 PMID: 14904456
  3. Linkage map of Salmonella typhimurium, Edition VI.
    Microbiol Rev. 1983 Sep;47(3):410-53 PMID: 6314110
  4. Determination of the rates of synthesis and degradation of adenosine 3',5'-cyclic monophosphate in Escherichia coli CRP- and CRP+ strains.
    Can J Biochem. 1978 Sep;56(9):849-52 PMID: 215288
  5. Genetic regulation: the Lac control region.
    Science. 1975 Jan 10;187(4171):27-35 PMID: 1088926
  6. Effects of crp mutations on adenosine 3',5'-monophosphate metabolism in Salmonella typhimurium.
    J Bacteriol. 1976 Jul;127(1):120-7 PMID: 179973
  7. Cyclic adenosine 3',5'-monophosphate in Escherichia coli.
    J Bacteriol. 1973 Jun;114(3):1068-73 PMID: 4351386
  8. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  9. D-Mannitol utilization in Salmonella typhimurium.
    J Bacteriol. 1971 Jan;105(1):232-40 PMID: 4322346
  10. A phage P22 gene controlling integration of prophage.
    Virology. 1967 Feb;31(2):207-16 PMID: 6021093
  11. Regulation of the synthesis of adenylate cyclase in Escherichia coli by the cAMP -- cAMP receptor protein complex.
    Mol Gen Genet. 1981;181(4):470-5 PMID: 6267421
  12. Cyclic 3', 5'-adenosine monophosphate phosphodiesterase mutants of Salmonella typhimurium.
    J Bacteriol. 1975 Jun;122(3):1081-90 PMID: 168178
  13. Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene.
    Nucleic Acids Res. 1983 Jun 11;11(11):3451-65 PMID: 6344011
  14. Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region.
    EMBO J. 1983;2(5):791-7 PMID: 6357786
  15. Regulation of histidine operon does not require hisG enzyme.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5021-5 PMID: 1108009
  16. Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.
    Nature. 1981 Apr 30;290(5809):744-9 PMID: 6261152
  17. Cyclic AMP as a modulator of polarity in polycistronic transcriptional units.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3194-7 PMID: 226959
  18. Regulation of synthesis of a major outer membrane protein: cyclic AMP represses Escherichia coli protein III synthesis.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5520-3 PMID: 230479
  19. Reversal of mutator phage Mu integration.
    J Mol Biol. 1975 Jul 25;96(1):87-99 PMID: 1099217
  20. Interaction of cAMP receptor protein with the ompA gene, a gene for a major outer membrane protein of Escherichia coli.
    FEBS Lett. 1981 Jun 15;128(2):186-90 PMID: 7021177
  21. Cyclic adenosine monophosphate in bacteria.
    Science. 1970 Jul 24;169(3943):339-44 PMID: 4317896
  22. Hfr formation directed by tn10.
    Genetics. 1979 Apr;91(4):639-55 PMID: 17248903
  23. Cyclic nucleotides in bacteria.
    Adv Cyclic Nucleotide Res. 1976;7:1-48 PMID: 188312
  24. Spectral and kinetic evidence for two early electron acceptors in photosystem I.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):770-3 PMID: 16592621
  25. Adenosine 3':5'-cyclic monophosphate control of the enzymes of glutamine metabolism in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2922-6 PMID: 4404145
  26. Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).
    J Bacteriol. 1980 Sep;143(3):1325-31 PMID: 6773928
  27. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  28. Mechanism of activation of catabolite-sensitive genes: a positive control system.
    Proc Natl Acad Sci U S A. 1970 May;66(1):104-10 PMID: 4320461
  29. DNA sequence of the araBAD promoter in Escherichia coli B/r.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4724-8 PMID: 368797
  30. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants.
    J Bacteriol. 1978 Jan;133(1):149-57 PMID: 201606
  31. Cyclic nucleotides in procaryotes.
    Microbiol Rev. 1981 Dec;45(4):620-42 PMID: 6276705
  32. The cyclic 3',5'-adenosine monophosphate receptor protein and regulation of cyclic 3',5'-adenosine monophosphate synthesis in Escherichia coli.
    Mol Gen Genet. 1978 Sep 20;165(1):47-56 PMID: 213702
  33. Purification and DNA-binding properties of the catabolite gene activator protein.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1222-5 PMID: 4331084
  34. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli.
    Biochem Biophys Res Commun. 1969 Sep 24;37(1):151-7 PMID: 4310317
  35. Mapping of the chl-B gene in Salmonella typhimurium LT2.
    Mol Gen Genet. 1972;119(1):67-70 PMID: 4564717
  36. Regulation of adenylate cyclase synthesis in Escherichia coli: studies with cya-lac operon and protein fusion strains.
    J Bacteriol. 1982 Sep;151(3):1346-57 PMID: 6286596
  37. Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters.
    Cell. 1977 Nov;12(3):847-54 PMID: 200371
  38. Cyclic adenosine 5'-monophosphate in Escherichia coli.
    Bacteriol Rev. 1976 Sep;40(3):527-51 PMID: 186018
  39. Multiple regulation of the activity of adenylate cyclase in Escherichia coli.
    Mol Gen Genet. 1982;185(2):262-8 PMID: 6283317
  40. Cyclic 3',5'-adenosine monophosphate phosphodiesterase of Escherichia coli.
    J Bacteriol. 1973 Nov;116(2):857-66 PMID: 4355491
  41. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):809-13 PMID: 1093171
  42. Complex glnA-glnL-glnG operon of Escherichia coli.
    J Bacteriol. 1982 Apr;150(1):202-13 PMID: 6120929
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.2.641-649.1985
Published
1985-02-00
Pages
641-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214931
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.161.2.641-649.1985 PMC Full Text View journal details
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