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PMID: 2983311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 3 ·1985-02-00 ·Pages 699-702

Moore DD, Marks AR, Buckley DI, Kapler G, Payvar F, Goodman HM

Abstract

Glucocorticoid receptor (GCR) protein stimulates transcription from a variety of cellular genes. We show here that GCR partially purified from rat liver binds specifically to a site within the first intron of the human growth hormone (hGH) gene, approximately 100 base pairs downstream from the start of hGH transcription. GCR binding is selectively inhibited by methylation of two short, symmetrically arranged clusters of guanine residues within this site. A cloned synthetic 24-base-pair deoxyoligonucleotide containing the predicted GCR binding sequence interacts specifically with GCR. The hGH binding site shares sequence homology with a GCR binding site upstream from the human metallothionein II gene and a subset of GCR binding sites from mouse mammary tumor virus. All of these binding sites for this eukaryotic transcriptional regulatory protein show remarkable similarity in overall geometry to the binding sites for several prokaryotic transcriptional regulatory proteins.

MeSH Terms
Animals Base Sequence Binding Sites Chromosome Mapping Cloning, Molecular DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Restriction Enzymes/metabolism Filtration Growth Hormone/genetics Humans Liver/analysis Oligonucleotides/metabolism Rats Receptors, Glucocorticoid/metabolism Receptors, Steroid/metabolism Transcription, Genetic
Chemicals
Oligonucleotides Receptors, Glucocorticoid Receptors, Steroid Growth Hormone DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Moore D D
Marks A R
Buckley D I
Kapler G
Payvar F
Goodman H M
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.3.699
Published
1985-02-00
Pages
699-702
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397113
Subset
IM
Databases
GENBANK
J00148, K00612
External Links
PubMed source DOI: 10.1073/pnas.82.3.699 PMC Full Text View journal details
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