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PMID: 2985539 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene.

Journal of bacteriology ·Vol. 162 ·No. 2 ·1985-05-00 ·Pages 615-20

Lee CH, Hu ST, Swiatek PJ, Moseley SL, Allen SD, So M

Abstract

The Escherichia coli heat-stable enterotoxin STII gene in P307 is flanked by inverted repeat sequences, suggesting that the STII gene is part of a transposon. To study the transposability, a DNA fragment containing the putative STII transposon has been cloned. Results of transposition assays indicated that the STII gene can transpose from one plasmid to another. The size of the transposon has been determined to be approximately 9 kilobases. The structure and the location of the STII gene in clinical isolates of Escherichia coli have been investigated by restriction enzyme analyses. The structural genes of STII from different clinical isolates appear to be uniform in size, but the flanking sequences are heterogeneous. This result suggests that the STII genes in different isolates are not on the same transposon as observed in P307.

MeSH Terms
Bacterial Toxins DNA Transposable Elements DNA, Bacterial/genetics Enterotoxins/genetics Escherichia coli/genetics,pathogenicity Escherichia coli Proteins Genes, Bacterial Plasmids
Chemicals
Bacterial Toxins DNA Transposable Elements DNA, Bacterial Enterotoxins Escherichia coli Proteins heat stable toxin (E coli)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee C H
Hu S T
Swiatek P J
Moseley S L
Allen S D
So M
References (26)
26 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Immunological study of the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae.
    Infect Immun. 1974 Mar;9(3):564-70 PMID: 4206029
  3. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.
    Infect Immun. 1974 Aug;10(2):320-7 PMID: 4368545
  4. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  5. Human diarrheal disease caused by enterotoxigenic Escherichia coli.
    Annu Rev Microbiol. 1975;29:333-53 PMID: 1101807
  6. Genetic and physical characteristics of an enterotoxin plasmid.
    J Bacteriol. 1975 Dec;124(3):1240-7 PMID: 1104580
  7. Deletions affecting the transposition of an antibiotic resistance gene.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):702-6 PMID: 322140
  8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  9. Heat-stable enterotoxin of Escherichia coli: in vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2800-4 PMID: 26915
  10. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2276-80 PMID: 276868
  11. Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.
    Infect Immun. 1978 Aug;21(2):526-31 PMID: 357288
  12. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1.
    Nature. 1979 Feb 8;277(5696):453-6 PMID: 368646
  13. Isolation and partial characterization of two different heat-stable enterotoxins produced by bovine and porcine strains of enterotoxigenic Escherichia coli.
    Infect Immun. 1979 Oct;26(1):173-7 PMID: 387603
  14. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  15. Purification and characterization of heat-stable enterotoxin produced by a strain of E. coli pathogenic for man.
    J Biol Chem. 1980 May 25;255(10):4716-21 PMID: 6989817
  16. Nucleotide sequence of the bacterial transposon Tn1681 encoding a heat-stable (ST) toxin and its identification in enterotoxigenic Escherichia coli strains.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4011-5 PMID: 6254008
  17. Structure of a naturally occurring plasmid with genes for enterotoxin production and drug resistance.
    J Bacteriol. 1981 Jan;145(1):97-105 PMID: 6257645
  18. Escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences.
    J Bacteriol. 1981 Feb;145(2):850-60 PMID: 6257652
  19. Comparison of enterotoxic activities of heat-stable enterotoxins from class 1 and class 2 Escherichia coli of swine origin.
    Infect Immun. 1981 Jan;31(1):245-51 PMID: 7011991
  20. Peptide toxins from Conus geographus venom.
    J Biol Chem. 1981 May 25;256(10):4734-40 PMID: 7014556
  21. Amino acid sequence of heat-stable enterotoxin produced by Escherichia coli pathogenic for man.
    J Biol Chem. 1981 Aug 10;256(15):7744-6 PMID: 7021541
  22. A study of the dissemination of Tn1681: a bacterial transposon encoding a heat-stable toxin among enterotoxigenic Escherichia coli isolates.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:53-8 PMID: 6271490
  23. Transposable elements in prokaryotes.
    Annu Rev Genet. 1981;15:341-404 PMID: 6279020
  24. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.
    Infect Immun. 1983 Mar;39(3):1167-74 PMID: 6341230
  25. Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers.
    Infect Immun. 1983 Oct;42(1):264-8 PMID: 6352495
  26. Nucleotide sequence of the gene for heat-stable enterotoxin II of Escherichia coli.
    Infect Immun. 1983 Oct;42(1):269-75 PMID: 6352496
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.162.2.615-620.1985
Published
1985-05-00
Pages
615-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218893
Subset
IM
Grants
NIAID NIH HHS · 1 RO1 AI21428-01 · United States
NCRR NIH HHS · 5 SO7 RR5371 · United States
External Links
PubMed source DOI: 10.1128/jb.162.2.615-620.1985 PMC Full Text View journal details
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