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PMID: 2986116 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2412-6

Hertzberg EL, Spray DC, Bennett MV

Abstract

Antibody raised against isolated rat liver gap junctions was microinjected into coupled cells in culture to assess its influence on gap junctional conductance. A rapid inhibition of fluorescent dye transfer and electrical coupling was produced in pairs of freshly dissociated adult rat hepatocytes and myocardial cells as well as in pairs of superior cervical ganglion neurons from neonatal rats cultured under conditions in which electrotonic synapses form. The antibodies have been shown by indirect immunofluorescence to bind to punctate regions of the plasma membrane in liver. By immunoreplica analysis of rat liver homogenates, plasma membranes, and isolated gap junctions resolved on NaDodSO4/polyacrylamide gels, binding was shown to be specific for the 27-kDa major polypeptide of gap junctions. This and similar antibodies should provide a tool for further investigation of the role of cell-cell communication mediated by gap junctions and indicate that immunologically similar polypeptides comprise gap junctions in adult mammalian cells derived from all three germ layers.

MeSH Terms
Animals Antibodies Cell Communication Connexins Electrophysiology Fluorescent Dyes Intercellular Junctions/physiology Isoquinolines Liver/physiology Membrane Proteins/immunology,physiology Rats
Chemicals
Antibodies Connexins Fluorescent Dyes Isoquinolines Membrane Proteins lucifer yellow
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hertzberg E L
Spray D C
Bennett M V
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.8.2412
Published
1985-04-00
Pages
2412-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397568
Subset
IM
Grants
NIGMS NIH HHS · GM 30667 · United States
NINDS NIH HHS · NS 16524 · United States
NINDS NIH HHS · NS 19830 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.8.2412 PMC Full Text View journal details
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