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PMID: 2987824 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.

Nucleic acids research ·Vol. 13 ·No. 5 ·1985-03-11 ·Pages 1431-42

Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P, Blanchard JM

Abstract

We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens DNA/analysis Endonucleases/metabolism Gene Expression Regulation Glyceraldehyde-3-Phosphate Dehydrogenases/genetics Humans Nucleic Acid Conformation RNA, Messenger/analysis Rats Rats, Inbred Strains Single-Strand Specific DNA and RNA Endonucleases
Chemicals
RNA, Messenger DNA Glyceraldehyde-3-Phosphate Dehydrogenases Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fort P
Marty L
Piechaczyk M
el Sabrouty S
Dani C
Jeanteur P
Blanchard J M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.5.1431
Published
1985-03-11
Pages
1431-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341087
Subset
IM
Databases
GENBANK
X02231
External Links
PubMed source DOI: 10.1093/nar/13.5.1431 PMC Full Text View journal details
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