A systematic study of HLA class II-beta DNA restriction fragments in insulin-dependent diabetes mellitus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3335-9

Cohen-Haguenauer O, Robbins E, Massart C, Busson M, Deschamps I, Hors J, Lalouel JM, Dausset J, Cohen D

Abstract

DNA restriction fragments of the genes encoding HLA class II-beta antigens were compared in 34 patients with insulin-dependent diabetes mellitus and 34 HLA-DR-matched healthy individuals. Ninety-three fragments, determined by six restriction enzymes (EcoRI, EcoRV, HindIII, BamHI, Pvu II, and Taq I), were analyzed: (i) A DR Taq I 12.7-kilobase-pair fragment might be a marker for the extended haplotype HLA-B8, DR3. (ii) In controls, DR4 haplotypes are associated with two distinct clusters of DQ restriction fragments (DQR4 and DQR5). Almost all (94%) DR4 patients belong to the DQR4 and not to the DQR5 cluster. This suggests that, among HLA-DR4 haplotypes, only DQR4 haplotypes are involved in susceptibility to insulin-dependent diabetes mellitus. (iii) A DR Taq I 14.5-kilobase-pair fragment was found to be strongly associated with DQR4, mainly in DR3/DR4 heterozygous patients (P = 5 X 10(-4). However, these results must be interpreted with caution, taking into account the high number of statistical tests performed.

MeSH Terms

DNA Restriction Enzymes Diabetes Mellitus, Type 1/genetics,immunology Genes HLA-DR Antigens Histocompatibility Antigens Class II/genetics Humans Polymorphism, Genetic Statistics as Topic

Chemicals

HLA-DR Antigens Histocompatibility Antigens Class II DNA Restriction Enzymes

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Cohen-Haguenauer O

Robbins E

Massart C

Busson M

Deschamps I

Hors J

Lalouel J M

Dausset J

Cohen D

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13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.10.3335

Published

1985-05-00

Pages

3335-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397770

Subset

External Links

PubMed source DOI: 10.1073/pnas.82.10.3335 PMC Full Text View journal details

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A systematic study of HLA class II-beta DNA restriction fragments in insulin-dependent diabetes mellitus.

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