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PMID: 2987922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Relative rates of homologous and nonhomologous recombination in transfected DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3355-9

Roth DB, Wilson JH

Abstract

Both homologous and nonhomologous recombination events occur at high efficiency in DNA molecules transfected into mammalian cells. Both types of recombination occur with similar overall efficiencies, as measured by an endpoint assay, but their relative rates are unknown. In this communication, we measure the relative rates of homologous and nonhomologous recombination in DNA transfected into monkey cells. This measurement is made by using a linear simian virus 40 genome that contains a 131-base-pair duplication at its termini. Once inside the cell, this molecule must circularize to initiate lytic infection. Circularization can occur either by direct, nonhomologous end-joining or by homologous recombination within the duplicated region. Although the products of the two recombination pathways are different, they are equally infectious. Since homologous and nonhomologous recombination processes are competing for the same substrate, the relative amounts of the products of each pathway should reflect the relative rates of homologous and nonhomologous recombination. Analysis of individual recombinant genomes from 164 plaques indicates that the rate of circularization by nonhomologous recombination is 2- to 3-fold higher than the rate of homologous recombination. The assay system described here may prove to be useful for testing procedures designed to influence the relative rates of homologous and nonhomologous recombination.

MeSH Terms
Animals Cells, Cultured Chlorocebus aethiops Gene Conversion Recombination, Genetic Repetitive Sequences, Nucleic Acid Simian virus 40/genetics Transfection
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roth D B
Wilson J H
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3355
Published
1985-05-00
Pages
3355-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397774
Subset
IM
Grants
NCI NIH HHS · CA 15743 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.10.3355 PMC Full Text View journal details
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