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PMID: 6587362 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recombination of transfected DNAs in vertebrate cells in culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 11 ·1984-06-00 ·Pages 3476-80

Bandyopadhyay PK, Watanabe S, Temin HM

Abstract

We studied the frequency of homologous and illegitimate recombinations between transfected noninfectious retroviral DNA molecules in chicken embryo fibroblasts. The frequency of recombination was determined by the formation of infectious virus and varied with the extent of homology between the DNA molecules at the site of recombination, but only when there were regions of homology surrounding this region. While homologous recombination led to the formation of wild-type virus, illegitimate recombination resulted in formation of infectious virus with alterations at the site of recombination. Apparent homologous recombination was also observed between transfected and chromosomal DNAs in D17 dog cells.

MeSH Terms
Animals Cells, Cultured Chick Embryo Cloning, Molecular DNA, Viral/genetics Recombination, Genetic Retroviridae/genetics Transfection
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bandyopadhyay P K
Watanabe S
Temin H M
References (28)
28 references, click to expand
  1. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  2. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors.
    Mol Cell Biol. 1983 Dec;3(12):2241-9 PMID: 6318091
  3. An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.
    J Gen Virol. 1976 Dec;33(3):447-58 PMID: 187721
  4. Recombination between endogenous and exogenous simian virus 40 genes. II. Biochemical evidence for genetic exchange.
    J Virol. 1977 Nov;24(2):541-50 PMID: 199740
  5. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  6. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  7. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  8. Insertion of a genetic marker into the ribosomal DNA of yeast.
    Plasmid. 1979 Oct;2(4):536-54 PMID: 394173
  9. Integration and excision of SV40 DNA from the chromosome of a transformed cell.
    Cell. 1980 May;20(1):143-52 PMID: 6248231
  10. Genetic and physical linkage of exogenous sequences in transformed cells.
    Cell. 1980 Nov;22(1 Pt 1):309-17 PMID: 6253083
  11. Expression of a transposable antibiotic resistance element in Saccharomyces.
    Nature. 1980 Oct 30;287(5785):869-71 PMID: 6253817
  12. Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.
    Nucleic Acids Res. 1980 Jun 25;8(12):2725-36 PMID: 6253890
  13. Indiscriminate recombination in simian virus 40-infected monkey cells.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4861-5 PMID: 6254043
  14. Recombination in SV40-infected cells: nucleotide sequences at viral-viral recombinant joints in naturally arising variants.
    Virology. 1981 Mar;109(2):344-52 PMID: 6259816
  15. Integrated simian virus 40 DNA: nucleotide sequences at cell-virus recombinant junctions.
    J Virol. 1981 May;38(2):671-9 PMID: 6264135
  16. Spontaneous changes in nucleotide sequence in proviruses of spleen necrosis virus, an avian retrovirus.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1230-4 PMID: 6951170
  17. Nucleotide sequence analysis of the recombinant joints in 16 naturally arising deletion mutants of simian virus 40.
    Virology. 1982 Dec;123(2):464-9 PMID: 6294985
  18. Expression of complete transplantation antigens by mammalian cells transformed with truncated class I genes.
    Nature. 1983 Feb 3;301(5899):388-94 PMID: 6823314
  19. High efficiency polyoma DNA transfection of chloroquine treated cells.
    Nucleic Acids Res. 1983 Mar 11;11(5):1295-308 PMID: 6298741
  20. Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2002-6 PMID: 6132383
  21. High-efficiency ligation and recombination of DNA fragments by vertebrate cells.
    Science. 1983 May 6;220(4597):606-9 PMID: 6301012
  22. Clustered illegitimate recombination events in mammalian cells involving very short sequence homologies.
    Nature. 1983 Jul 14-20;304(5922):181-4 PMID: 6306477
  23. Homologous and nonhomologous recombination in monkey cells.
    Mol Cell Biol. 1983 Jun;3(6):1040-52 PMID: 6308421
  24. Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4827-31 PMID: 6576360
  25. Generation of adenovirus by transfection of plasmids.
    Nucleic Acids Res. 1983 Sep 10;11(17):6003-20 PMID: 6310523
  26. Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5986-90 PMID: 6310558
  27. Homologous recombination between transfected DNAs.
    Mol Cell Biol. 1983 Sep;3(9):1680-5 PMID: 6314127
  28. Replication of reticuloendotheliosis viruses in cell culture: acute infection.
    J Virol. 1974 Feb;13(2):291-7 PMID: 4855738
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.11.3476
Published
1984-06-00
Pages
3476-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345531
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-22443 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.11.3476 PMC Full Text View journal details
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