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PMID: 2988941 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Eukaryotic type I topoisomerase is enriched in the nucleolus and catalytically active on ribosomal DNA.

The EMBO journal ·Vol. 4 ·No. 5 ·1985-05-00 ·Pages 1237-43

Muller MT, Pfund WP, Mehta VB, Trask DK

Abstract

The distribution of eukaryotic DNA topoisomerase I in the cell has been analyzed at four levels: (i) at the level of the nuclear matrix; (ii) at the cytological level by immunofluorescence of whole cells; (iii) at the electron microscopic level using the protein A/colloidal gold technique; and (iv) at the level of DNA to identify in situ the sequence upon which topoisomerase I is catalytically active. Although topoisomerase I is clearly distributed non-randomly in the nucleus, the unique distribution of the enzyme is not related to the nuclear matrix. The data support the conclusion that topoisomerase I is heavily concentrated in the nucleolus of the cell; furthermore, particular regions within the nucleolus are depleted of topoisomerase. A technique has been developed which allows isolation and analysis of the cellular DNA sequences covalently attached to topoisomerase. Ribosomal DNA sequences are at least 20-fold enriched in topoisomerase/DNA complexes isolated directly from a chromosomal setting, relative to total DNA. This is the first direct evidence that topoisomerase I is catalytically active on ribosomal DNA in vivo.

MeSH Terms
Animals Antibodies/immunology Base Sequence Cell Nucleolus/enzymology DNA Topoisomerases, Type I/analysis,immunology DNA, Ribosomal/metabolism Microscopy, Electron Rabbits Transcription, Genetic
Chemicals
Antibodies DNA, Ribosomal DNA Topoisomerases, Type I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Muller M T
Pfund W P
Mehta V B
Trask D K
References (38)
38 references, click to expand
  1. Varigated chromatin structures of mouse ribosomal RNA genes.
    J Mol Biol. 1983 Jun 15;167(1):133-55 PMID: 6864798
  2. Effect of intercalating agents on RNA polymerase I promoter selection in Xenopus laevis.
    Mol Cell Biol. 1984 Dec;4(12):2851-7 PMID: 6543244
  3. A site and strand specific nuclease activity with analogies to topoisomerase I frames the rRNA gene of Tetrahymena.
    Nucleic Acids Res. 1983 Nov 25;11(22):7661-78 PMID: 6316268
  4. DNA topoisomerases--enzymes that catalyse the breaking and rejoining of DNA.
    CRC Crit Rev Biochem. 1983;15(1):1-24 PMID: 6319080
  5. Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1431-5 PMID: 6369327
  6. Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II.
    EMBO J. 1984 Mar;3(3):671-6 PMID: 6325181
  7. Two cell lines from lymphomas of Marek's disease.
    Biken J. 1974 Sep;17(3):105-16 PMID: 4616680
  8. Simple procedure for isolation of DNA, RNA and protein fractions from cultured animal cells.
    Anal Biochem. 1975 May 12;65(1-2):125-31 PMID: 1130673
  9. Isolation of ribosomal gene chromatin.
    Dev Biol. 1977 Feb;55(2):375-86 PMID: 557006
  10. Ultrastructure and activity of the nucleolar organizer in the mouse oocyte during meiotic prophase.
    J Cell Sci. 1978 Jun;31:79-100 PMID: 670331
  11. Proteins that affect DNA conformation.
    Annu Rev Biochem. 1978;47:449-79 PMID: 209728
  12. Peripheral RNA synthesis of fibrillar center in nucleoli of Japanese quail oocytes and somatic cells.
    J Ultrastruct Res. 1978 Sep;64(3):377-87 PMID: 568671
  13. Nucleologenesis in chick erythrocyte nuclei reactivated by cell fusion.
    J Ultrastruct Res. 1979 Nov;69(2):164-79 PMID: 40045
  14. Nicking-closing activity associated with bacteriophage lambda int gene product.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3760-4 PMID: 226979
  15. Effect of protease inhibitors on matrix proteins and the association of replicating DNA.
    Exp Cell Res. 1979 Oct 15;123(2):411-4 PMID: 499368
  16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  17. Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break.
    Cell. 1980 Mar;19(3):697-707 PMID: 6244895
  18. DNA gyrase stimulates transcription.
    Nucleic Acids Res. 1980 Feb 25;8(4):845-54 PMID: 6253926
  19. DNA topoisomerases.
    Cell. 1980 Nov;22(2 Pt 2):327-8 PMID: 6256077
  20. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51 PMID: 6265907
  21. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  22. Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):56-60 PMID: 6948303
  23. Active chromatin.
    Nature. 1982 May 27;297(5864):289-95 PMID: 6210847
  24. Properties of active nucleosomes as revealed by HMG 14 and 17 chromatography.
    Nucleic Acids Res. 1982 Mar 25;10(6):2017-42 PMID: 6210882
  25. Non-histone proteins and long-range organization of HeLa interphase DNA.
    J Mol Biol. 1982 Apr 5;156(2):325-44 PMID: 6896344
  26. Nuclear lamina and the structural organization of the nuclear envelope.
    Cold Spring Harb Symp Quant Biol. 1982;46 Pt 2:967-78 PMID: 7049540
  27. Association of eukaryotic DNA topoisomerase I with nucleosomes and chromosomal proteins.
    Nucleic Acids Res. 1983 Jan 25;11(2):461-72 PMID: 6298726
  28. Biochemical characterization of topoisomerase I purified from avian erythrocytes.
    Nucleic Acids Res. 1983 May 11;11(9):2779-800 PMID: 6304657
  29. Regulation of DNA supercoiling in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:763-7 PMID: 6305584
  30. Localization of nucleolar phosphoproteins B23 and C23 during mitosis.
    Exp Cell Res. 1983 Jun;146(1):139-49 PMID: 6345184
  31. DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2616-20 PMID: 6326134
  32. Nucleolar nomenclature.
    J Cell Sci. 1984 Apr;67:217-20 PMID: 6746773
  33. Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4275-9 PMID: 6379641
  34. Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23.
    Chromosoma. 1984;90(2):139-48 PMID: 6206987
  35. Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin.
    J Mol Biol. 1984 Oct 5;178(4):897-919 PMID: 6092647
  36. Replacement synthesis labeling of DNA molecules in vitro using the Escherichia coli exonuclease III/DNA polymerase I enzyme pair.
    Anal Biochem. 1984 Aug 15;141(1):33-7 PMID: 6388409
  37. Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6958-62 PMID: 6095263
  38. Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.
    Cell. 1983 Aug;34(1):105-13 PMID: 6309403
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-05-00
Pages
1237-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554330
Subset
IM
Grants
NIGMS NIH HHS · R01 GM31640-02 · United States
External Links
PubMed source PMC Full Text View journal details
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