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PMID: 2989255 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Uncoupling of osmoregulation of the Escherichia coli K-12 ompF gene from ompB-dependent transcription.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 82-7

Ramakrishnan G, Ikenaka K, Inouye M

Abstract

The expression of the genes for the Escherichia coli K-12 outer membrane proteins, ompF and ompC, is subject to osmoregulation and responds to changes in the osmolarity of the growth medium. The transcription of these genes is dependent on the products of the regulatory locus ompB (comprising the genes ompR and envZ). The native promoter of ompF was replaced with an inducible lpp promoter to eliminate this transcriptional dependence of ompF expression on ompB. As a result, it was possible for the OmpF protein to be produced in an ompB mutant strain that does not normally express ompF. Surprisingly, the expression of ompF under the lpp promoter was still osmoregulated not only in the ompB+ strain but also in two ompB strains tested. These results indicate the involvement of a factor(s) besides the ompR and envZ gene products in the osmoregulation of OmpF production. This factor may interact with a sequence downstream of the ompF promoter. In addition, we show that the expression of ompF under the lpp promoter has no direct effect on ompC expression.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics DNA Transposable Elements Escherichia coli/genetics,physiology Gene Expression Regulation Operon Plasmids Promoter Regions, Genetic Transcription, Genetic Water-Electrolyte Balance
Chemicals
Bacterial Outer Membrane Proteins DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ramakrishnan G
Ikenaka K
Inouye M
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.82-87.1985
Published
1985-07-00
Pages
82-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219083
Subset
IM
Grants
NIGMS NIH HHS · GM19043 · United States
External Links
PubMed source DOI: 10.1128/jb.163.1.82-87.1985 PMC Full Text View journal details
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