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PMID: 2989655 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposon mutagenesis and genetic mapping of the rglA and rglB loci of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 198 ·No. 3 ·1985-00-00 ·Pages 390-2

Ravi RS, Sozhamannan S, Dharmalingam K

Abstract

The rglA and rglB genes code for two different proteins which cleave the hydroxymethylated cytosine residues of T-even phages. We isolated Tn10 and Tn5 insertion mutants of the above genes and of the genes in and around the rglA and rglB loci. These insertions were used to construct a detailed genetic map. Our results show that the rglA gene maps at 25.24 min and the rglB gene at 98.39 min on the standard Escherichia coli K12 genetic map.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ravi R S
Sozhamannan S
Dharmalingam K
References (13)
13 references, click to expand
  1. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
    Genetics. 1978 Nov;90(3):427-61 PMID: 365678
  2. Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli.
    Mol Gen Genet. 1969;105(4):285-97 PMID: 4904136
  3. Genetic mapping and transcriptional orientation of the fimD gene.
    J Bacteriol. 1983 Dec;156(3):1052-8 PMID: 6139362
  4. Mechanism localisation and control of restriction cleavage of phage T4 and lambda chromosomes in vivo.
    Nature. 1976 Apr 1;260(5550):406-10 PMID: 768782
  5. A model for three-point analysis of random general transduction.
    Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
  6. Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12.
    J Bacteriol. 1979 Jun;138(3):726-30 PMID: 110766
  7. Mapping of the structural genes of the three aspartokinases and of the two homoserine dehydrogenases of Escherichia coli K-12.
    J Bacteriol. 1974 Jan;117(1):133-43 PMID: 4148765
  8. Genetics of host-controlled restriction and modification in Escherichia coli.
    Genet Res. 1969 Apr;13(2):227-40 PMID: 4894745
  9. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12.
    Virology. 1967 Apr;31(4):688-701 PMID: 4290282
  10. On the structure of the glucosylated hydroxymethylcytosine nucleotides of coliphages T2, T4, and T6.
    J Biol Chem. 1960 Nov;235:3254-9 PMID: 13760441
  11. chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.
    J Bacteriol. 1983 Mar;153(3):1513-20 PMID: 6298188
  12. Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR.
    J Bacteriol. 1980 May;142(2):621-32 PMID: 6247326
  13. DNA-glucosylation in T-even phage: genetic determination and role in phagehost interaction.
    Annu Rev Genet. 1970;4(0):177-92 PMID: 4950809
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1985-00-00
Pages
390-2
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
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