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PMID: 6139362 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic mapping and transcriptional orientation of the fimD gene.

Journal of bacteriology ·Vol. 156 ·No. 3 ·1983-12-00 ·Pages 1052-8

Freitag CS, Eisenstein BI

Abstract

Expression of type 1 fimbriae in Escherichia coli exhibits phase variation whereby individual cells can alternate between states of organelle expression and nonexpression. Strains carrying fim-lac operon fusions in which lac operon expression is under the control of a fim promoter undergo Lac+ in equilibrium Lac- phase variation. We have determined the genetic map location and direction of transcription of a fim-lac operon fusion which was obtained by insertion of lac into a locus we have named fimD. We found the gene order to be as follows: valS fimD uxuA serB. The direction of transcription of fimD was found to be clockwise on the E. coli chromosome.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements DNA, Recombinant Escherichia coli/genetics,ultrastructure Fimbriae, Bacterial/metabolism Genes, Bacterial Mutation Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Recombinant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Freitag C S
Eisenstein B I
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.3.1052-1058.1983
Published
1983-12-00
Pages
1052-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217949
Subset
IM
Grants
NIAID NIH HHS · AI-19670 · United States
External Links
PubMed source DOI: 10.1128/jb.156.3.1052-1058.1983 PMC Full Text View journal details
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