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PMID: 2989775 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

rRNA processing: removal of only nineteen bases at the gap between 28S alpha and 28S beta rRNAs in Sciara coprophila.

Nucleic acids research ·Vol. 13 ·No. 10 ·1985-05-24 ·Pages 3581-97

Ware VC, Renkawitz R, Gerbi SA

Abstract

We have determined the sequence of the rDNA region between the 28S alpha and 28S beta rRNA coding segments (termed a "gap") in the insect Sciara coprophila, and have used S1 nuclease mapping and cDNA primer extension to define the 5' and 3' boundaries of the gap. Only 19 bases found in rDNA at the gap region are absent from mature 28S rRNA. Eukaryotic rRNAs contain stretches of nucleotides ("expansion segments") which are absent in E. coli rRNA. The gap region in Sciara is located within expansion segment V. Therefore, the excision of 19 bases in the Sciara gap suggests that a large portion of expansion segment V plays no function in mature ribosomes. Specific sequences conserved in Sciara and Drosophila are considered as candidates for recognition signals for the excision of the gap transcript.

MeSH Terms
Animals Base Sequence Biological Evolution Diptera/genetics Drosophila/genetics Endonucleases/pharmacology Models, Structural RNA Processing, Post-Transcriptional RNA, Ribosomal/analysis Single-Strand Specific DNA and RNA Endonucleases Tetrahymena/genetics
Chemicals
RNA, Ribosomal Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ware V C
Renkawitz R
Gerbi S A
References (35)
35 references, click to expand
  1. Dissociation of ribonucleic acid from silkmoth pupae by heat and dimethylsulfoxide: evidence for specific cleavage points.
    J Mol Biol. 1966 Oct 28;21(1):29-41 PMID: 5338995
  2. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  3. Characterization of a new low molecular weight RNA in HeLa cell ribosomes.
    J Mol Biol. 1968 May 14;33(3):609-23 PMID: 5700415
  4. Studies on the 3'-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with "hidden" breaks in heart-dissociable insects 26S ribonucleic acid.
    Biochem J. 1974 Sep;141(3):617-25 PMID: 4219141
  5. Occurrence in vivo of "hidden breaks" at specific sites of 26 S ribosomal RNA of Musca carnaria.
    J Mol Biol. 1975 Jun 15;95(1):9-20 PMID: 1152058
  6. The fragments from the 28 S ribosomal RNA of Galleria mellonella with unesterified uridine at the 3'-termini.
    Biochim Biophys Acta. 1976 Mar 4;425(2):185-95 PMID: 175853
  7. Late steps in the maturation of Drosophila 26 S ribosomal RNA: generation of 5-8 S and 2 S RNAs by cleavages occurring in the cytoplasm.
    J Mol Biol. 1976 Feb 15;101(1):85-105 PMID: 768488
  8. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
    Cell. 1977 Feb;10(2):177-92 PMID: 402221
  9. The structural organization of ribosomal DNA in Drosophila melanogaster.
    Cell. 1977 Feb;10(2):193-212 PMID: 402222
  10. Sequence arrangement of the rDNA of Drosophila melanogaster.
    Cell. 1977 Feb;10(2):213-4 PMID: 402223
  11. Increase in hepatic tyrosine aminotransferase mRNA during enzyme induction by N6,O2'-dibutyryl cyclic AMP.
    J Biol Chem. 1978 Jan 25;253(2):319-22 PMID: 22549
  12. Structure of psoralen-crosslinked ribosomal RNA from Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1642-6 PMID: 417342
  13. Introduction of hidden breaks during rRNA maturation and ageing in Tetrahymena pyriformis.
    Eur J Biochem. 1978 Jul 3;87(3):607-16 PMID: 98329
  14. Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments.
    J Mol Biol. 1978 Dec 25;126(4):749-68 PMID: 106128
  15. Ribosomal DNA of fly Sciara coprophila has a very small and homogeneous repeat unit.
    Mol Gen Genet. 1979 May 23;173(1):1-13 PMID: 288964
  16. The intervening sequence in the 26S rRNA coding region of T. thermophila is transcribed within the largest stable precursor for rRNA.
    Cell. 1979 Oct;18(2):525-32 PMID: 498282
  17. Fine structure of ribosomal RNA. II. Distribution of methylated sequences within Xenopus laevis rRNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1497-511 PMID: 388355
  18. Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5051-5 PMID: 291921
  19. Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
    Nucleic Acids Res. 1979 Dec 20;7(8):2213-38 PMID: 118436
  20. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):201-4 PMID: 6153795
  21. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  22. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  23. Coding and spacer sequences in the 5.8S-2S region of Sciara coprophila ribosomal DNA.
    Nucleic Acids Res. 1980 Aug 25;8(16):3565-73 PMID: 7433100
  24. The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.
    Nucleic Acids Res. 1981 Jun 25;9(12):2853-69 PMID: 7024906
  25. Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.
    Nucleic Acids Res. 1981 Aug 11;9(15):3747-64 PMID: 7279671
  26. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
    Cell. 1981 Nov;27(1 Pt 2):165-74 PMID: 7326749
  27. The primary and secondary structure of yeast 26S rRNA.
    Nucleic Acids Res. 1981 Dec 21;9(24):6935-52 PMID: 7335496
  28. The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae.
    Nucleic Acids Res. 1981 Dec 21;9(24):6953-8 PMID: 6460984
  29. Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.
    Cell. 1982 Nov;31(1):147-57 PMID: 6297745
  30. Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3903-7 PMID: 6306649
  31. Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA.
    Nucleic Acids Res. 1983 Sep 10;11(17):5903-20 PMID: 6193488
  32. [Sequence of the central break region of the precursor of Drosophila 26S ribosomal RNA].
    C R Seances Acad Sci III. 1983;296(22):1041-4 PMID: 6313147
  33. Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis.
    Nucleic Acids Res. 1983 Nov 25;11(22):7795-817 PMID: 6359063
  34. Xenopus laevis 28S ribosomal RNA: a secondary structure model and its evolutionary and functional implications.
    Nucleic Acids Res. 1984 Aug 10;12(15):6197-220 PMID: 6147812
  35. Secondary structure of the circular form of the Tetrahymena rRNA intervening sequence: a technique for RNA structure analysis using chemical probes and reverse transcriptase.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):648-52 PMID: 2579378
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.10.3581
Published
1985-05-24
Pages
3581-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341260
Subset
IM
Grants
NIGMS NIH HHS · PHS-GM 20261 · United States
Databases
GENBANK
X02482
External Links
PubMed source DOI: 10.1093/nar/13.10.3581 PMC Full Text View journal details
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