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PMID: 7279671 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3747-64

Renkawitz-Pohl R, Matsumoto L, Gerbi SA

Abstract

We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual ribosomal DNA organization in this organism were studied in detail. Clone pBc 1L-1 has an intervening sequence of 1.4 kb, and clone pBc 6D-6 has an intervening sequence of 0.9 kb. These intervening sequences occur in about the same position in 28S rDNA, but do not appear to share sequence homology with one another. Previously we found that 90% of Sciara ribosomal DNA is homogenous and lacks an intervening sequence, and our present data explains the size heterogeneity found in most of the remaining 10%. We have found no evidence of size heterogeneity in the nontranscribed spacer.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/isolation & purification DNA Replication DNA, Recombinant Diptera/genetics Male Microscopy, Electron Nucleic Acid Conformation Nucleic Acid Hybridization Ribosomes/analysis Transcription, Genetic
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Renkawitz-Pohl R
Matsumoto L
Gerbi S A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3747
Published
1981-08-11
Pages
3747-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327389
Subset
IM
Grants
NIGMS NIH HHS · GM 00036 · United States
NIGMS NIH HHS · GM 22882 · United States
NIGMS NIH HHS · GM 26070 · United States
External Links
PubMed source DOI: 10.1093/nar/9.15.3747 PMC Full Text View journal details
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