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PMID: 372737 Published · ppublish English Journal Article

Inserted sequence in the mitochondrial 23S ribosomal RNA gene of the yeast Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 168 ·No. 1 ·1979-01-05 ·Pages 101-9

Faye G, Dennebouy N, Kujawa C, Jacq C

Abstract

The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead to the conclusion that the 23S rRNA1 gene of the omega+ strains is split by an insertion sequence of 1,000-1,100 bp. In contrast, no detactable insertion was found in the 23S rRNA gene of the omega- strains. The size and the location of the insert found in the 23S rRNA gene of the omega+ strains appear to be identical to those of the sequence delta which had previously been found to characterize the difference (at the omega locus) between the mitDNA of the wild type strains carrying the omega+ or omega- alleles (Jacq et al., 1977).

MeSH Terms
Base Sequence DNA, Circular/analysis,genetics DNA, Mitochondrial/analysis,genetics Genes Genetic Linkage RNA, Ribosomal/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics
Chemicals
DNA, Circular DNA, Mitochondrial RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Faye G
Dennebouy N
Kujawa C
Jacq C
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33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267939
Published
1979-01-05
Pages
101-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267939
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