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PMID: 2990892 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.

The EMBO journal ·Vol. 4 ·No. 1 ·1985-01-00 ·Pages 179-84

Dowhan W, Bibus CR, Schatz G

Abstract

Yeast cytochrome c oxidase contains three large subunits made in mitochondria and at least six smaller subunits made in the cytoplasm. There is evidence that the catalytic centers (heme a and copper) are associated with the mitochondrially-made subunits, but the role of the cytoplasmically-made subunits has remained open. Using a gene interruption technique, we have now constructed a Saccharomyces cerevisiae mutant which lacks the largest of the cytoplasmically-made subunits (subunit IV). This mutant is devoid of cyanide-sensitive respiration, the absorption spectrum of cytochrome aa3 and cytochrome c oxidase activity. It still contains the other cytochrome c oxidase subunits but these are not assembled into a stable complex. Active cytochrome c oxidase was restored to the mutant by introducing a plasmid-borne wild-type subunit IV gene; no restoration was seen with a gene carrying an internal deletion corresponding to amino acid residues 28-66 of the mature subunit. Subunit IV is thus necessary for proper assembly of cytochrome c oxidase.

MeSH Terms
Cytoplasm/metabolism Electron Transport Complex IV/genetics Extrachromosomal Inheritance Genes Mutation Saccharomyces cerevisiae/genetics
Chemicals
Electron Transport Complex IV
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dowhan W
Bibus C R
Schatz G
References (34)
34 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-01-00
Pages
179-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554168
Subset
IM
Grants
NIGMS NIH HHS · GM 20478 · United States
NIGMS NIH HHS · GM 25047 · United States
External Links
PubMed source PMC Full Text View journal details
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