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PMID: 29912 Published · ppublish English Journal Article

The stimulus-secretion coupling of glucose-induced insulin release. Metabolic and functional effects of NH4+ in rat islets.

The Journal of clinical investigation ·Vol. 62 ·No. 4 ·1978-10-00 ·Pages 868-78

Sener A, Hutton JC, Kawazu S, Boschero AC, Somers G, Devis G, Herchuelz A, Malaisse WJ

Abstract

NH4+ caused a dose-related, rapid, and reversible inhibition of glucose-stimulated insulin release by isolated rat islets. It also inhibited glyceraldehyde-, Ba2+-, and sulfonylurea-stimulated insulun secretion. NH4+ failed to affect glucose utilization and oxidation, glucose-stimulated proinsulin biosynthesis, the concentration of ATP, AD, and AMP, and the intracellular pH. NH4+ also failed to affect the ability of theophylline and cytochalasin B to augment glucose-induced insulin release. However, in the presence and absence of glucose, accumulation of NH4+ in islet cells was associated with a fall in the concentration of NADH and HADPH and a concomitant alteration of 86Rb+ and 45Ca2+ (or 133Ba2+) handling. These findings suggest that reduced pyridine nucleotides, generated by the metabolism of endogenous of exogenous nutrients, may modulate ionophoretic processes in the islet cells and by doing so, affect the net uptake of Ca2+ and subsequent release of insulin.

MeSH Terms
Ammonia/pharmacology Animals Cations/metabolism Female Glucose/antagonists & inhibitors,metabolism Glyceraldehyde/antagonists & inhibitors Hydrogen-Ion Concentration Insulin/metabolism Insulin Secretion Islets of Langerhans/drug effects,metabolism Proinsulin/biosynthesis Pyridines/metabolism Rats Water/metabolism
Chemicals
Cations Insulin Pyridines Water Glyceraldehyde Ammonia Proinsulin Glucose
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sener A
Hutton J C
Kawazu S
Boschero A C
Somers G
Devis G
Herchuelz A
Malaisse W J
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI109199
Published
1978-10-00
Pages
868-78
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC371839
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI109199 PMC Full Text View journal details
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