Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 199 ·No. 3 ·1985-00-00 ·Pages 446-51

Catcheside DE, Storer PJ, Klein B

Abstract

We have constructed a phage, lambda Ncl, which comprises a 4.0 kb HindIII insert of Neurospora DNA into the immunity region of the vector lambda 598. lambda Ncl complements the aroD6 mutation of E. coli, permitting the formation of galaxy plaques on medium lacking aromatic supplements, and transforms an aro-9 qa-2 Neurospora mutant to prototrophy at a low frequency. Low levels of 5-dehydroquinate hydrolyase (E.C.4.2.1.10.), with properties unlike those of the catabolic isoenzyme that is coded by qa-2, are present in E. coli aroD6 cell lysates following infection with lambda Ncl. lambda Ncl does not hybridize with qa-2 DNA and it is concluded that it contains at least the aro-9 region of the pentafunctional aro cluster gene.

MeSH Terms

Amino Acids/genetics Bacteriophage lambda/genetics Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics Genes Genes, Fungal Genetic Vectors Genotype Hydro-Lyases/genetics Neurospora/genetics Neurospora crassa/genetics Nucleic Acid Hybridization Transcription, Genetic

Chemicals

Amino Acids DNA Transposable Elements Hydro-Lyases 3-dehydroquinate dehydratase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Catcheside D E

Storer P J

Klein B

References (24)

24 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330757

Published

1985-00-00

Pages

446-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330757

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Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli.

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