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PMID: 2994016 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.

Nucleic acids research ·Vol. 13 ·No. 16 ·1985-08-26 ·Pages 5869-82

Furutani Y, Notake M, Yamayoshi M, Yamagishi J, Nomura H, Ohue M, Furuta R, Fukui T, Yamada M, Nakamura S

Abstract

DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Escherichia coli/genetics Humans Interleukin-1/genetics Macrophages/metabolism Nucleic Acid Hybridization Plasmids Protein Precursors/genetics RNA, Messenger/genetics Rabbits Species Specificity
Chemicals
Interleukin-1 Protein Precursors RNA, Messenger interleukin 1 precursor DNA DNA Restriction Enzymes
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Furutani Y
Notake M
Yamayoshi M
Yamagishi J
Nomura H
Ohue M
Furuta R
Fukui T
Yamada M
Nakamura S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.16.5869
Published
1985-08-26
Pages
5869-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321918
Subset
IM
Databases
GENBANK
X02851, X02852
External Links
PubMed source DOI: 10.1093/nar/13.16.5869 PMC Full Text View journal details
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