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PMID: 2997134 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Evidence that polygalacturonase is a virulence determinant in Erwinia carotovora.

Journal of bacteriology ·Vol. 164 ·No. 2 ·1985-11-00 ·Pages 831-5

Lei SP, Lin HC, Heffernan L, Wilcox G

Abstract

Polygalacturonase (PG) was purified from Erwinia carotovora EC. A hybrid cosmid, pSH711, that encodes PG activity but not pectate lyase activity was identified from an E. carotovora genomic library by an immunological screening method. A cell extract of Escherichia coli cells containing pSH711 was able to produce plant tissue maceration when spotted on carrot, potato, or turnip slices. In addition, the E. coli strain containing this plasmid was able to macerate carrot, potato, and turnip slices. Our results suggest that PG plays an important role in soft-rot disease.

MeSH Terms

Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease HindIII Erwinia/enzymology Escherichia coli/genetics Genes Genes, Bacterial Glycoside Hydrolases/metabolism Plant Diseases Plants/metabolism Polygalacturonase/genetics,isolation & purification,metabolism

Chemicals

DNA Restriction Enzymes Deoxyribonuclease HindIII Glycoside Hydrolases Polygalacturonase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lei S P

Lin H C

Heffernan L

Wilcox G

References (13)

13 references, click to expand

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Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes.
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Rapid and efficient cosmid cloning.
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The genus Erwinia: enterobacteria pathogenic to plants and animals.
Annu Rev Microbiol. 1972;26:389-426 PMID: 4562815
Donor strains of the soft-rot bacterium Erwinia chrysanthemi and conjugational transfer of the pectolytic capacity.
J Bacteriol. 1977 Dec;132(3):862-9 PMID: 924974
In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.
EMBO J. 1985 Mar;4(3):787-92 PMID: 16453607
Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.
Arch Biochem Biophys. 1968 Feb;123(2):298-306 PMID: 5642600
The araC regulatory gene mRNA contains a leader sequence.
Mol Gen Genet. 1980;180(1):219-26 PMID: 6160371
Molecular cloning in Escherichia coli of Erwinia chrysanthemi genes encoding multiple forms of pectate lyase.
J Bacteriol. 1985 Mar;161(3):913-20 PMID: 2982794
Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.
Gene. 1985;35(1-2):63-70 PMID: 3896936
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
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Plasmids of Escherichia coli as cloning vectors.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.164.2.831-835.1985

Published

1985-11-00

Pages

831-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214326

Subset

Grants

NIGMS NIH HHS · GM30491 · United States

External Links

PubMed source DOI: 10.1128/jb.164.2.831-835.1985 PMC Full Text View journal details

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Evidence that polygalacturonase is a virulence determinant in Erwinia carotovora.

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