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PMID: 2997742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences.

Nucleic acids research ·Vol. 13 ·No. 20 ·1985-10-25 ·Pages 7171-82

McClelland M, Nelson M, Cantor CR

Abstract

The restriction modification methylase M. Mbo II has been purified using a sensitive oligonucleotide linker assay. The enzyme methylates the Mbo II recognition sequence* GAAGA at adenine to produce GAAGmA. M. Mbo II can be used in conjunction with the methylation dependent restriction endonuclease Dpn I (GmATC) to produce cleavage at the 10 base sequence GAAGATCTTC. When M. Mbo II is used in combination with M. Cla I (ATCGATCGAT), cleavage by Dpn I occurs at the four ten base sequences GAAGATCTTC, GAAGATCGAT, ATCGATCTTC and ATCGATCGAT, which is equivalent to a nine base recognition site. The use of combinations of adenine methylases and Dpn I to generate highly selective DNA cleavages at a variety of sequences up to fourteen base pairs is discussed.

MeSH Terms
Base Sequence DNA Restriction Enzymes Methylation Methyltransferases/isolation & purification Moraxella/enzymology Site-Specific DNA-Methyltransferase (Adenine-Specific) Substrate Specificity
Chemicals
DNA modification methylase MboII Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McClelland M
Nelson M
Cantor C R
References (16)
16 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.20.7171
Published
1985-10-25
Pages
7171-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322036
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.20.7171 PMC Full Text View journal details
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