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PMID: 6278447 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6795-804

McClelland M

Abstract

A method for detecting Type II modification methylases and determining their methylation site by assaying the ability of methylated DNA to be cleaved by heterologous restriction enzymes is described and applied to the isolation of the restriction modification methylases from Thermus thermophilus HB8, Thermus aquaticus YTI and Caryophanon latum L. M.TaqI is shown to have a methylation specificity identical to M.ThI (TCGmeA). M.ClaI methylates at adenine and protects a subset of TthI sites indicating that it methylates the sequence ATCGmeAT. Methylation by M.ThI also protects against cleavage by SalI, XhoI and at some HindII, AccI and MboI sites.

MeSH Terms

Adenine Bacteria/enzymology Base Sequence DNA Restriction Enzymes Methyltransferases/isolation & purification Site-Specific DNA-Methyltransferase (Adenine-Specific) Substrate Specificity Thermus/enzymology

Chemicals

DNA modification methylase MClaI DNA modification methylase MTaqI Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) DNA Restriction Enzymes Adenine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

McClelland M

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.24.6795

Published

1981-12-21

Pages

6795-804

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327642

Subset

Grants

NCRR NIH HHS · RF 10-21-RR093-009 · United States

External Links

PubMed source DOI: 10.1093/nar/9.24.6795 PMC Full Text View journal details

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Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

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