Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.

Nucleic acids research ·Vol. 13 ·No. 20 ·1985-10-25 ·Pages 7183-94

Sawadaishi K, Miura K, Ohtsuka E, Ueda T, Ishizaki K, Shinriki N

Abstract

Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites.

MeSH Terms

Chemical Phenomena Chemistry Chromosome Mapping DNA Restriction Enzymes DNA, Bacterial DNA, Circular DNA, Superhelical Hydrazines Ozone Plasmids

Chemicals

DNA, Bacterial DNA, Circular DNA, Superhelical Hydrazines hydrazine Ozone DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Sawadaishi K

Miura K

Ohtsuka E

Ueda T

Ishizaki K

Shinriki N

References (16)

16 references, click to expand

Hairpin-loop formation by inverted repeats in supercoiled DNA is a local and transmissible property.
Nucleic Acids Res. 1981 Mar 25;9(6):1271-89 PMID: 6262723
Cruciform structures in supercoiled DNA.
Nature. 1981 Feb 5;289(5797):466-70 PMID: 7464915
Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase.
Anal Biochem. 1981 May 1;113(1):34-42 PMID: 6168210
Degradation of nucleic acids with ozone. II. Degradation of yeast RNA, yeast phenylalanine tRNA and tobacco mosaic virus RNA.
Biochim Biophys Acta. 1981 Oct 27;655(3):323-8 PMID: 7025912
Dynamic, sequence-dependent DNA structure as exemplified by cruciform extrusion from inverted repeats in negatively supercoiled DNA.
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:101-12 PMID: 6305553
Effects of salts, temperature, and stem length on supercoil-induced formation of cruciforms.
J Biol Chem. 1983 Jun 25;258(12):7661-8 PMID: 6863259
Cruciform-resolvase interactions in supercoiled DNA.
Cell. 1984 Feb;36(2):413-22 PMID: 6319022
Osmium tetroxide: a new probe for site-specific distortions in supercoiled DNAs.
Nucleic Acids Res. 1984 Feb 10;12(3):1725-35 PMID: 6322118
Degradation of nucleic acids with ozone. III. Mode of ozone-degradation of mouse proline transfer ribonucleic acid (tRNA) and isoleucine tRNA.
Chem Pharm Bull (Tokyo). 1983 Oct;31(10):3601-8 PMID: 6561072
Changes in site specificity of single-strand-specific endonucleases on supercoiled PM2 DNA with temperature and ionic environment.
Nucleic Acids Res. 1984 Sep 25;12(18):7071-86 PMID: 6091053
Degradation of nucleic acids with ozone. V. Mechanism of action of ozone on deoxyribonucleoside 5'-monophosphates.
Chem Pharm Bull (Tokyo). 1984 Sep;32(9):3601-6 PMID: 6525654
Degradation of nucleic acids with ozone. VI. Labilization of the double-helical structure of calf thymus deoxyribonucleic acid.
Chem Pharm Bull (Tokyo). 1984 Sep;32(9):3636-40 PMID: 6525657
Sedimentation velocity behavior of closed circular SV40 DNA as a function of superhelix density, ionic strength, counterion and temperature.
J Mol Biol. 1971 Nov 28;62(1):21-38 PMID: 4333614
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72 PMID: 6256738
Degradation of nucleic acids with ozone. I. Degradation of nucleobases, ribonucleosides and ribonucleoside-5'-monophosphates.
Chem Pharm Bull (Tokyo). 1981 Mar;29(3):868-72 PMID: 7249159

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.20.7183

Published

1985-10-25

Pages

7183-94

Language

English

Region

England

NLM ID

0411011

PMCID

PMC322037

Subset

External Links

PubMed source DOI: 10.1093/nar/13.20.7183 PMC Full Text View journal details

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Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.

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