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PMID: 2998768 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural prerequisites of simian virus 40 large T antigen for the maintenance of cell transformation.

The EMBO journal ·Vol. 4 ·No. 11 ·1985-11-00 ·Pages 2941-7

Montenarh M, Kohler M, Aggeler G, Henning R

Abstract

We have investigated the functional roles of two structural subsets of simian virus 40 (SV40) large T antigen, namely homo-oligomers and complexes with the host cellular p53 protein, for the transformed phenotype. We examined T antigen produced in cells transformed by temperature-sensitive SV40 large T mutants: heat-sensitive or unrestricted SV40 tsA58-transformed rat cells and unrestricted tsA1499 transformants. In both unrestricted cell lines, T antigen was temperature-sensitive only for the formation of fast sedimenting homo-oligomers. Corresponding to our recent observations obtained with tsA1499-infected monkey cells, in tsA1499 transformants large T was competent to form stable T-p53 complexes independently of the temperature. However, T antigen coded for by tsA58, which is heat-sensitive for binding to p53, occurred in stable complexes with this protein in unrestricted tsA58 transformants under all conditions. Furthermore, in both unrestricted transformants T-p53 complexes arise in the absence of homo-oligomers of T antigen. In conclusion, T antigen homo-oligomers are not involved in cell transformation, whereas T-p53 complexes may be involved in the maintenance of this phenotype.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics,isolation & purification Cell Line Cell Transformation, Neoplastic Cricetinae Mesocricetus Mutation Oncogene Proteins, Viral/genetics,isolation & purification Phenotype Protein Kinases/genetics Simian virus 40/enzymology,genetics Temperature
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Montenarh M
Kohler M
Aggeler G
Henning R
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47 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-11-00
Pages
2941-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554602
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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