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PMID: 2999080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the DNA region encoding nodulation functions in Bradyrhizobium japonicum.

Journal of bacteriology ·Vol. 164 ·No. 3 ·1985-12-00 ·Pages 1301-8

Russell P, Schell MG, Nelson KK, Halverson LJ, Sirotkin KM, Stacey G

Abstract

The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a number of overlapping recombinant clones from this region allowed the construction of a restriction map of the region. The identified nodulation region of B. japonicum shows homology exclusively to those regions of R. meliloti and Rhizobium leguminosarum DNA known to encode early nodulation functions. The region of homology with these two fast-growing Rhizobium species was narrowed to an 11.7-kilobase segment. A nodulation-defective mutant of Rhizobium fredii USDA 201, strain A05B-2, was isolated and found to be defective in the ability to curl soybean root hairs. Some of the isolated recombinant DNA clones of B. japonicum were found to restore wild-type nodulation function to this mutant. Analysis of the complementation results allows the identification of a 1.8-kilobase region as essential for restoration of Hac function.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Bacterial/isolation & purification Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Electrophoresis, Agar Gel Genetic Complementation Test Mutation Nucleic Acid Hybridization Rhizobiaceae/genetics
Chemicals
DNA, Bacterial DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Russell P
Schell M G
Nelson K K
Halverson L J
Sirotkin K M
Stacey G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.3.1301-1308.1985
Published
1985-12-00
Pages
1301-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219330
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.164.3.1301-1308.1985 PMC Full Text View journal details
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