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PMID: 2999081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, structure, and expression of the Escherichia coli K-12 hisC gene.

Journal of bacteriology ·Vol. 164 ·No. 3 ·1985-12-00 ·Pages 1317-23

Grisolia V, Carlomagno MS, Nappo AG, Bruni CB

Abstract

We used an expression vector plasmid containing the Escherichia coli K-12 histidine operon regulatory region to subclone the E. coli hisC gene. Analysis of plasmid-coded proteins showed that hisC was expressed in minicells. A protein with an apparent molecular weight of 38,500 was identified as the primary product of the hisC gene. Expression was under control of the hisGp promoter and resulted in very efficient synthesis (over 100-fold above the wild-type levels) of imidazolylacetolphosphate:L-glutamate aminotransferase, the hisC gene product. The complete nucleotide sequence of the hisC gene has been determined. The gene is 1,071 nucleotides long and codes for a protein of 356 amino acids with only one histidine residue.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes/metabolism Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Histidine Molecular Weight Operon Plasmids
Chemicals
Bacterial Proteins Histidine DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grisolia V
Carlomagno M S
Nappo A G
Bruni C B
References (56)
56 references, click to expand
  1. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  2. Amino acid sequence of ATP phosphoribosyltransferase of Salmonella typhimurium.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1589-92 PMID: 377278
  3. Model for regulation of the histidine operon of Salmonella.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):508-12 PMID: 6987654
  4. Structural and physiological studies of the Escherichia coli histidine operon inserted into plasmid vectors.
    J Bacteriol. 1980 Apr;142(1):32-42 PMID: 6246067
  5. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Cloning and restriction map of the first part of the histidine operon of Salmonella typhimurium.
    J Bacteriol. 1981 Jul;147(1):124-34 PMID: 6263863
  8. In vivo and in vitro detection of the leader RNA of the histidine operon of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2767-71 PMID: 6166940
  9. Genetic analysis of the histidine operon control region of Salmonella typhimurium.
    J Mol Biol. 1981 Feb 5;145(4):713-34 PMID: 7021855
  10. DNA sequence changes of mutations altering attenuation control of the histidine operon of Salmonella typhimurium.
    J Mol Biol. 1981 Feb 5;145(4):735-56 PMID: 6167727
  11. Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues.
    J Mol Biol. 1981 Feb 15;146(1):45-54 PMID: 7021857
  12. Identification, nucleotide sequence and expression of the regulatory region of the histidine operon of Escherichia coli K-12.
    Nucleic Acids Res. 1981 May 11;9(9):2075-86 PMID: 6170941
  13. Chemical and genetic studies on L-histidinol dehydrogenase of Salmonella typhimurium. Isolation and structure of the tryptic peptides.
    J Mol Biol. 1981 Apr 15;147(3):451-64 PMID: 7031260
  14. Regulation of the histidine operon: translation-controlled transcription termination (a mechanism common to several biosynthetic operons).
    Curr Top Cell Regul. 1981;19:1-45 PMID: 6277571
  15. The complete nucleotide sequence of the tryptophan operon of Escherichia coli.
    Nucleic Acids Res. 1981 Dec 21;9(24):6647-68 PMID: 7038627
  16. Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12.
    J Bacteriol. 1982 Aug;151(2):692-700 PMID: 6284708
  17. Internal promoters of the his operon in Salmonella typhimurium.
    J Bacteriol. 1983 Feb;153(2):1114-9 PMID: 6296044
  18. Codon usage in bacteria: correlation with gene expressivity.
    Nucleic Acids Res. 1982 Nov 25;10(22):7055-74 PMID: 6760125
  19. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  20. Structure and function of the internal promoter (hisBp) of the Escherichia coli K-12 histidine operon.
    J Bacteriol. 1983 Sep;155(3):1288-96 PMID: 6309747
  21. Gene organization in the distal part of the Salmonella typhimurium histidine operon and determination and sequence of the operon transcription terminator.
    Mol Gen Genet. 1983;191(3):413-20 PMID: 6314092
  22. Use of M13mp phages to study gene regulation, structure and function: cloning and recombinational analysis of genes of the Salmonella typhimurium histidine operon.
    Gene. 1983 Dec;26(2-3):147-58 PMID: 6323256
  23. Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors.
    J Biol Chem. 1984 Jun 25;259(12):7399-403 PMID: 6736011
  24. The hisD-hisC gene border of the Salmonella typhimurium histidine operon.
    Mol Gen Genet. 1984;196(3):526-9 PMID: 6390096
  25. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  26. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  27. Isolation of the fourth (isomerase) of histidine biosynthesis from Salmonella typhimurium.
    J Biol Chem. 1966 Jul 25;241(14):3262-9 PMID: 5330429
  28. Physical and chemical characterization of the isomerase of histidine biosynthesis in Salmonella typhimurium.
    J Biol Chem. 1967 Jan 25;242(2):256-64 PMID: 4959618
  29. Imidazolylacetolphosphate:L-glutamate aminotransferase. Purification and physical properties.
    J Biol Chem. 1967 Mar 25;242(6):1168-74 PMID: 5337155
  30. Imidazolylacetolphosphate:L-glutamate aminotransferase. Composition and substructure.
    J Biol Chem. 1967 Mar 25;242(6):1175-81 PMID: 5337156
  31. Purification and composition studies of phosphoribosyladenosine triphosphate:pyrophosphate phosphoribosyltransferase, the first enzyme of histidine biosynthesis.
    J Biol Chem. 1967 Apr 25;242(8):1760-7 PMID: 5337591
  32. Polarity in the histidine operon.
    J Mol Biol. 1966 Nov 14;21(2):357-69 PMID: 4291404
  33. Translation and polarity in the histidine operon. II. Polarity in the histidine operon.
    J Mol Biol. 1967 Nov 28;30(1):97-107 PMID: 4294872
  34. Histidinol dehydrogenase from Salmonella typhimurium. Crystallization and composition studies.
    J Biol Chem. 1968 Jun 25;243(12):3264-72 PMID: 4872176
  35. Composition and subunit structure of histidinol dehydrogenase from Salmonella typhimurium.
    J Biol Chem. 1968 Jun 25;243(12):3277-88 PMID: 4872178
  36. Transcription initiation in the histidine operon of Salmonella typhimurium.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):925-32 PMID: 4909470
  37. Imidazolylacetolphosphate: L-glutamate aminotransferase-mechanism of action.
    Arch Biochem Biophys. 1970 May;138(1):239-44 PMID: 4911025
  38. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  39. Enzyme evolution: generation of a bifunctional enzyme by fusion of adjacent genes.
    Nature. 1970 Nov 28;228(5274):820-4 PMID: 4920435
  40. Properties of a fused protein formed by genetic manipulation. Histidinol dehydrogenase-imidazolylacetol phosphate: L-glutamate aminotransferase.
    J Biol Chem. 1971 Mar 25;246(6):1806-13 PMID: 4323234
  41. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  42. An intercistronic region in the histidine operon of Salmonella typhimurium.
    J Mol Biol. 1972 Aug 28;69(3):427-52 PMID: 4562711
  43. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  44. Regulation of the expression of the histidine operon in Salmonella typhimurium.
    Nature. 1974 Jun 7;249(457):523-7 PMID: 4599761
  45. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  46. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  47. Internal promoter P2 of the histidine operon of Salmonella typhimurium.
    J Bacteriol. 1974 Nov;120(2):984-6 PMID: 4616955
  48. Histidinol dehydrogenase from salmonella typhimurium and Escherichia coli. Purification, some characteristics and the amino acid sequence around a reactive thiol group.
    Biochim Biophys Acta. 1977 Aug 23;493(2):429-40 PMID: 329890
  49. A simple, general procedure for purifying restriction endonucleases.
    Nucleic Acids Res. 1977 Aug;4(8):2561-72 PMID: 909783
  50. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  51. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  52. Linkage map of Salmonella typhimurium, edition V.
    Microbiol Rev. 1978 Jun;42(2):471-519 PMID: 353483
  53. Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4276-80 PMID: 360215
  54. DNA sequence from the histidine operon control region: seven histidine codons in a row.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4281-5 PMID: 360216
  55. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  56. pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene.
    Gene. 1980 Feb;8(3):239-53 PMID: 6244214
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.3.1317-1323.1985
Published
1985-12-00
Pages
1317-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219332
Subset
IM
Databases
GENBANK
K00054, M11982, X03416
External Links
PubMed source DOI: 10.1128/jb.164.3.1317-1323.1985 PMC Full Text View journal details
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