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PMID: 2999795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a polymorphic variant associated with HLA-DQw3 and characterized by specific restriction sites within the DQ beta-chain gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 8139-43

Kim SJ, Holbeck SL, Nisperos B, Hansen JA, Maeda H, Nepom GT

Abstract

Restriction endonuclease digestion of genomic DNA from 24 lymphoblastoid cell lines homozygous for the HLA class II specificity DQw3, followed by hybridization with a DQ beta-chain cDNA probe, identified a genomic polymorphism with variable BamHI and HindIII recognition sites. This restriction fragment pattern was found for several haplotypes associated with the DQw3 specificity, including some haplotypes positive for the HLA-DR specificities DR4, DR5, DRw8, and DRw12. The variant fragment pattern corresponds precisely with the reactivity of a monoclonal antibody, A-10-83, previously shown to define a serologic split of DQw3. Serologic detection of the specific DQw3.1 genomic polymorphism indicated that the corresponding DQ beta-chain variants are expressed. This polymorphic restriction fragment pattern, then, represents a selective marker for DQ beta-chain genes that appear to define a DQ beta-chain-associated specificity, here called DQw3.1.

MeSH Terms
Antibodies, Monoclonal DNA Restriction Enzymes Diabetes Mellitus, Type 1/genetics Genes HLA-DQ Antigens Histocompatibility Antigens Class II/genetics,immunology Humans Polymorphism, Genetic
Chemicals
Antibodies, Monoclonal HLA-DQ Antigens Histocompatibility Antigens Class II DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kim S J
Holbeck S L
Nisperos B
Hansen J A
Maeda H
Nepom G T
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.8139
Published
1985-12-00
Pages
8139-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391458
Subset
IM
Grants
NCI NIH HHS · CA18029 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.23.8139 PMC Full Text View journal details
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